安徽农业科学2016，Vol.44Issue(33)：143-144,181,3.
生石花植株离体再生及组培快繁研究
Plantlet Regeneration in vitro and Rapid Propagation of Lithops sp.
摘要
Abstract
Objective] The aim was to establish plantlet regeneration in vitro and rapid propagation of Lithops sp.[Method] With mature seeds as explant, rapid propagation technique of Lithops sp.was studied.[ Result] Calli were induced from the young seedling on MS medium +0.50 mg/L 6-BA+0.05 mg/L NAA in this study.The results showed that the basic MS medium without 6-BA benefited the callus differentiation of Lithops sp., and the induction ratio of adventitious bud was 4.65;MS+0.05 mg/L 6-BA+0.01 mg/L NAA was the optimal medium for the multiplication of adventitious bud, and the multiplication ratio was 5.60.[Conclusion] The plantlet regeneration and rapid propagation system of Lithops sp.were established, which has important guiding significance for the germplasm protection and industrial production of Lithops sp..关键词
生石花/愈伤组织/离体再生/增殖/生根Key words
Lithops sp./Callus/Regeneration in vitro/Proliferation/Rooting分类
农业科技引用本文复制引用
牟豪杰,王燕,吕永平,汪一婷,李海营..生石花植株离体再生及组培快繁研究[J].安徽农业科学,2016,44(33):143-144,181,3.基金项目
引进国际先进农业科学技术计划（948）项目（2011－G31）；浙江省农业科学院青年人才培养项目（2015R21R08E08）。 （）
