吉林医药学院学报2017，Vol.38Issue(1)：7-11,5.
结核分枝杆菌RegX3基因表达情况及其蛋白的生物信息学分析
Analysis of RegX3 gene expression and its protein bioinformation in Myco-bacterium tuberculosis
陈璐 1李冰洁2
作者信息
- 1. 南京市浦口区中心医院检验科，江苏南京 211800
- 2. 江苏大学医学院检验系，江苏镇江 212013
- 折叠
摘要
Abstract
Objective To study RegX3 gene expression of Mycobacterium tuberculosis ( MTB) and to predict the structure and function of its coding protein by bioinformatics technology .Methods MTB strains were isolated from clinical patients and RegX3 gene was amplificated by PCR and sequenced ,then analysed its gene expression and poly-morphism.The structure and function of RegX3 protein was analyzed by bioinformatics technology .Results Positive rate of RegX3 gene was 65 .9%( 27/41 ) from clinical patients .Sequencing results showed that the 11 .1%( 3/27 ) RegX3 gene occur base insertion ,deletion and substitution .Bioinformatics analysis showed that RegX 3 protein belong to the stable protein,it's hydrophilicity was better than hydrophobic .In the secondary structure of RegX3 protein,alpha helix and random curl were the majority ,and it had eight phosphorylation sites .Conclusion RegX3 gene was highly conservative .Bioinformatics analysis of RegX 3 protein provides guidance for further research protein function .关键词
结合分枝杆菌RegX3基因/基因多态性/生物信息学Key words
Mycobacterium tuberculosis RegX3 gene/gene polymorphism/bioinformatics analysis分类
生物科学引用本文复制引用
陈璐,李冰洁..结核分枝杆菌RegX3基因表达情况及其蛋白的生物信息学分析[J].吉林医药学院学报,2017,38(1):7-11,5.
