吉林医药学院学报2017，Vol.38Issue(1)：12-14,3.
冻存对经血源性间充质干细胞表面标记的影响
The effect of cryopreservation on the expression of cell membrane marker on Menstrual blood-derived mesenchymal stem cells
摘要
Abstract
Objective To analysisthe change of 3 cell surface markers on menstrual blood-derived mesenchymal stem cells ( MMCs) and study the expression of cell membrane markers on menstrual blood-derived mesenchymal stem cells affacted by cryopreservation .Methods The mononuclear cells were separated from menstrual blood by lymphocyte separation medium .After primary and continuous cell culture , the high proliferating subgroup was obtained from the mononuclear cells.The cells morphology was observed by microscope and the cell surface markers (CD105,CD14 and CD45) were measured by flow cytometry before or after cryopreservation .Results The MMCs liked could be ob-tained from menstrual blood mononuclear cells after 3 times continuous culturing .It was found that these MMCs ex-pressed high level CD105,but the expression of CD14 or CD45 was weak.The cryopreservation had no influenceon cell phenotype of MMCs .Conclusion The MMCs could be obtained from menstrual blood , and the cell phenotype CD105high,CD14low,CD45low do not been affacted by cryopreservation.关键词
冻存/经血源性/间充质干细胞/细胞表标记Key words
cryopreservation/Menstrual blood-derived/mesenchymal stem cells/surface marker分类
医药卫生引用本文复制引用
王静静,张丽,沈芝颖,胡婷,郭素红,李妍..冻存对经血源性间充质干细胞表面标记的影响[J].吉林医药学院学报,2017,38(1):12-14,3.基金项目
吉林省大学生创新课题；吉林省卫生厅科研课题（2011Z089）；吉林省教育厅科研课题（2012483）． （）
