吉林医药学院学报2017，Vol.38Issue(1)：18-20,3.
RP-HPLC法同时测定小儿肺热咳喘颗粒中两种主要活性成分的含量
Simultaneuos determination of two main component of Xiao'er Feire Kech-uan Granules by RP-HPLC
摘要
Abstract
Objective To establish a method for simultaneous determination of chlorogenic acid and baicalin in Xiao'er Feire Kechuan Granules .Methods Using PLATSILTM ODS (250 mm ×4.6 mm,5μm) column with metha-nol (A) -0.1%phosphoric acid (B) gradient:0 ~5 min,35%~50%A;5~10 min,50%~75%A;10~17 min, 75%~90%A;17~25 min,90%A;25~30 min,90%~35%A;30~40 min,35%A,a flow rate of 1 mL/min,detec-tion wavelength:327 nm, the column temperature was 30 ℃.Results The results showed that the linear range of chlorogenic acid and baicalin was 0.084~1.260μg (R=0.999 5) and 0.242~3.630μg (R=0.9996) respec-tively ,and the sample amout and the peak area had a good linear relationship .Conclusion The method is accurate , simple,with good reproducibility and stability ,and is suitable for quality control of Xiao'er Feire Kechuan Granules .关键词
HPLC/小儿肺热咳喘颗粒/绿原酸/黄芩苷Key words
HPLC/Xiao'er Feire Kechuan Granules/chlorogenic acid/baicalin分类
医药卫生引用本文复制引用
郝乘仪,张慧锋,杨明慧..RP-HPLC法同时测定小儿肺热咳喘颗粒中两种主要活性成分的含量[J].吉林医药学院学报,2017,38(1):18-20,3.基金项目
吉林省教育厅资助项目（2015379）． （）
