动物营养学报2016，Vol.28Issue(12)：91-98,8.DOI:10.3969/j.issn.1006-267x.2016.12.013
补喂瘤胃液制备物对羔羊肠道黏膜及血浆中免疫球蛋白含量的影响
Effects of Oral Administration of Ruminal Fluid Preparations on Immunoglobulin Contents in Intestinal Mucosa and Plasma of Lambs
摘要
Abstract
This experiment aimed to study the effects of oral administration of ruminal fluid preparations on im⁃munoglobulin contents in the intestinal mucosa and plasma of lambs, to explore the effects of ruminal fluid preparations on intestinal mucosal and humoral immunity of neonatal lambs. Fifty newborn lambs with similar birth weight as model animal were divided into 5 groups with 10 lambs per group. Lambs in trial groups were fed different preparations of ruminal fluid [ ruminal fluid (Ⅰ) , autoclaved ruminal fluid (Ⅱ) , ultrasonic pro⁃cessing ruminal fluid (Ⅲ) and autoclaved ultrasonic processing ruminal fluid (Ⅳ) ] from healthy adult sheep since 1 day of age once a day over a period of 5 days, and those in control group were given the same volume of sterile saline. Three lambs per group were slaughtered to obtain small intestinal tissues on 24 days of age;plasma was collected at 14 and 28 days of age, respectively. Immunoglobulin contents in intestinal mucosa and plasma were determined. The results showed as follows:1) in intestinal mucosal protein, the contents of im⁃munoglobulin (IgA), secretory immunoglobin A (SIgA) and immunoglobulin G (IgG) in all trial groups were higher than those in control group. The total content of IgA in trial groupⅢ was significantly higher than that in control group ( P<0.01) , as well as significantly higher than that in the other trial groups ( P<0.05);there were no significant differences of the total contents of SIgA and IgG among groups ( P>0.05) . In gener⁃ally, the contents of immunoglobulins showed ileum>duodenum>jejunum, and compared with control group, the total contents of immunoglobulins in trial group Ⅲ were greatly increased. 2) In plasma, immunoglobulin contents at 28 days of age were higher than those at 14 days of age. There was no significant difference of IgA content among groups at 14 days of age (P>0.05), however, at 28 days of age, the content of IgA in trial group Ⅰ was significantly increased compared with control group ( P<0.05) , and was significantly increased compared with the other trial groups ( P<0.01); there was no significant difference of the content of IgG a⁃mong groups at 14 and 28 days of age (P>0.05). In conclusion, orally administered different ruminal fluid preparations can improve intestinal mucosal immunity of newborn lambs, and ultrasonic processing ruminal flu⁃id shows better effects than the other preparations.关键词
瘤胃液制备物/新生羔羊/肠道黏膜/血浆/免疫球蛋白Key words
ruminal fluid preparation/newborn lamb/intestinal mucosa/plasma/immunoglobulin分类
农业科技引用本文复制引用
吴婷婷,韩冰,聂彪彪,董伟伟,杨开伦..补喂瘤胃液制备物对羔羊肠道黏膜及血浆中免疫球蛋白含量的影响[J].动物营养学报,2016,28(12):91-98,8.基金项目
国家自然科学基金 （）
