动物营养学报2016，Vol.28Issue(12)：108-115,8.DOI:10.3969/j.issn.1006-267x.2016.12.015
利用体外产气法研究3个品种柱花草与王草间的组合效应
A Study on Associative Effects of Three Cultivars of Stylosanthes with King Grass Using in Vitro Gas Production Technique
摘要
Abstract
This experiment was carried out to investigate the associative effects of three cultivars of stylosanthes with king grass using in vitro gas production technique. Three cultivars of stylosanthes ( S. guianensis Sw. Rey⁃an No. 2, S. guianensis Sw. Reyan No. 20 and S. guianensis Sw. Reyan No. 21) were combined with Pennis⁃etum purpureum×P.americanum cv.Reyan No. 4 (king grass) in different proportions (0∶100, 10∶90, 20∶80, 40∶60, 50∶50 and 100∶0) , respectively. The effects of combinations at different proportions on gas produc⁃tion, gas production parameters, in vitro dry matter digestibility ( IVDMD) , and ammonia nitrogen ( NH3⁃N) and microbial protein ( MCP) contents were analyzed using in vitro gas production technique. The single⁃fac⁃tors associative effects value and synthetically associative effects value of each combination were calculated to select the best proportions. The results showed as follows:1) significant differences were found in gas produc⁃tion of rapidly fermented fraction among different proportions of combinations of S. guianensis Sw. Reyan No. 2 and S. guianensis Sw. Reyan No. 20 with king grass ( P<0.05) , which reached the highest at proportions of 30∶70 and 50∶50, respectively. IVDMD reached the highest when S. guianensis Sw. Reyan No. 2∶king grass and S. guianensis Sw. Reyan No. 20∶king grass were 50∶50 and 40:60, respectively, and were significantly different from that of king grass only (P<0.05). MCP content reached the highest when S. guianensis Sw. Reyan No. 2∶king grass and S. guianensis Sw. Reyan No. 20∶king grass were 30∶70. NH3⁃N content tended to be firstly increased and then decreased with the increase of stylosanthes proportion, and all of the combinations reached the highest at 30∶70. 2) Synthetically associative effects value of three cultivars of stylosanthes with king grass all showed unimodal increase with the increase of stylosanthes proportion, and reached the highest when S. guianensis Sw. Reyan No. 2∶king grass and S. guianensis Sw. Reyan No. 20∶king grass were 30∶70, and S. guianensis Sw. Reyan No. 21∶king grass was 20∶80. In conclusion, the supplementation of proper stylosanthes in king grass can improve gas production, IVDMD, MCP content and ruminal microbial activity, and acts positively associative effects; the effects are the best when S. guianensis Sw. Reyan No. 2∶king grass and S. guianensis Sw. Reyan No. 20∶king grass and S. guianensis Sw. Reyan No. 21∶king grass are 30∶70, 30∶70 and 20∶80, respectively.关键词
柱花草/王草/体外产气/组合效应Key words
stylosanthes/king grass/in vitro gas production/associative effects分类
农业科技引用本文复制引用
张亚格,李茂,周汉林,胡琳,李韦,徐铁山..利用体外产气法研究3个品种柱花草与王草间的组合效应[J].动物营养学报,2016,28(12):108-115,8.基金项目
中国热带农业科学院基本科研业务费（1630032015044）；国家重点基础研究发展计划课题 （）
