动物营养学报2016，Vol.28Issue(12)：126-135,10.DOI:10.3969/j.issn.1006-267x.2016.12.017
意大利蜜蜂工蜂幼虫饲粮中适宜泛酸水平
Appropriate Dietary Pantothenic Acid Level for Apis mellifera ligustica Worker Bee Larvae
摘要
Abstract
The objective of this experiment was to research the influences of dietary pantothenic acid level on development, body antioxidant function and the expressions of CoA synthesis related enzyme genes for Apis mellifera ligustica worker bee larvae, and to find the appropriate dietary pantothenic acid level in this stage. A total of 1 800 one⁃day⁃old Apis mellifera ligustica worker bee larvae were randomly assigned to 5 groups with 3 replicates per group and 120 larvae per replicate. Larvae in 5 groups were fed with experimental diets with 0.92, 1.22, 1.52, 1.82 and 2.12 mg/g ( measured values) pantothenic acid until pupate, respectively. The body weight, body composition, haemolymph biochemical indices, antioxidant indices and the relative expres⁃sion levels of CoA synthesis related enzyme genes of 5⁃and 7⁃day⁃old larvae were measured, and then the pu⁃pation and eclosion rates were calculated. The results showed as follows:1) pantothenic acid supplementation could significantly increase the fresh weight, dry weight and ether extract ( EE) content of larvae ( P<0.05) , and eclosion rate in 2.12 mg/g group was significantly higher than that in other groups ( P<0.05) . 2) Dietary pantothenic acid level had significantly influences on total protein ( TP) , total cholesterol ( TCHO) , high den⁃sity lipoprotein ( HDL) and low density lipoprotein ( LDL) contents in haemolymph of 5⁃day⁃old larvae ( P<0.05), meanwhile, they reached the minimum in 1.82, 1.82, 1.22 and 1.52 mg/g groups, respectively. 3) The total anti⁃oxidant capacity ( T⁃AOC) of 5⁃and 7⁃day⁃old larvae was significantly increased with the dietary pantothenic acid level increasing ( P<0.05) , as well as the superoxide dismutase ( SOD) activity of 5⁃day⁃old larvae. 4) Dietary pantothenic acid level significantly influenced the relative expression levels of pantothenate kinase 4 ( PANK4 ) and phosphopantothenoylcysteine decarboxylase ( PPCDC ) of 5⁃day⁃old larvae ( P<0.05) , and both of them reached the maximum in 1.52 mg/g group. Considering dry weight and eclosion rate of 5⁃day⁃old larvae to make fitting curves, the appropriate dietary pantothenic acid level for Apis mellifera ligu⁃stica worker bee larvae is 1.85 to 2.01 mg/g.关键词
泛酸/意大利蜜蜂/工蜂/幼虫/适宜水平Key words
pantothenic acid/Apis mellifera ligustica/worker bee/larvae/appropriate level分类
农业科技引用本文复制引用
雷春红,马兰婷,王红芳,胥保华..意大利蜜蜂工蜂幼虫饲粮中适宜泛酸水平[J].动物营养学报,2016,28(12):126-135,10.基金项目
国家蜂产业体系建设专项资金（CARS-45）；山东省农业良种工程项目 （）
