动物营养学报2016，Vol.28Issue(12)：174-184,11.DOI:10.3969/j.issn.1006-267x.2016.12.022
硒源与硒水平对军曹鱼幼鱼生长性能、肝脏和血清抗氧化指标及组织硒含量的影响
Effects of Selenium Source and Selenium Level on Growth Performance, Liver and Serum Antioxidant Indices and Selenium Content in Tissues of Juvenile Cobia (Rachycentron canadum)
摘要
Abstract
A feeding trial was carried out to investigate the effects of selenium ( Se ) source and Se level on growth performance, liver and serum antioxidant indices and Se content in tissues of juvenile cobia ( Rachycen⁃tron canadum) , in order to determine the Se requirement from different Se sources of juvenile cobia. Nine ex⁃perimental diets (control diet was shared) were prepared by supplementing 0 (control), 0.3, 0.6, 0.9 and 1.2 mg/kg Se from sodium selenite ( Se⁃S) or selenium methionine ( Se⁃Met) , respectively. Juvenile cobia with an initial body weight of (22.18±0.35) g were fed to satiation for 10 weeks. Fish in three cages (repli⁃cates) were fed a kind of experimental diet and 30 fish in a cage. The results showed as follows:1) Se level extremely significantly affected the weight gain rate ( WGR) and specific growth rate ( SGR) ( P<0.01) , but did not significantly affected the feed conversion ratio ( FCR) and survival rate ( SR) ( P>0.05) . The SGR, WGR, FCR and SR were not affected by Se source and the interaction of Se source and Se level (P>0.05). With the Se level increasing, the SGR and WGR were increased firstly and then decreased. 2) The glutathione peroxidase ( GSH⁃Px) , glutathione reductase ( GR) activities and malondialdehyde ( MDA) content in liver and GSH⁃Px activity in serum were extremely significantly affected by Se level ( P<0.01) , and GR activity in serum was significantly affected by Se level ( P<0.05) . The GR, total superoxide dismutase ( T⁃SOD) , cata⁃lase ( CAT) activities and MDA content in liver and T⁃SOD activity in serum were extremely significantly af⁃fected by Se source ( P<0.01) . The interaction of Se level and Se source had significant effects on liver CAT activity, MDA content and serum T⁃SOD activity (P<0.05). With the Se level increasing, the activity of GSH⁃Px in liver and serum was increased firstly and then stabilized, and the GR activity in liver and serum was firstly decreased and then stabilized. The highest GSH⁃Px activity and the lowest GR activity in liver were ob⁃tained at the 0.9 mg/kg added level from two Se sources. The CAT activity in liver was increased with the Se level increasing. 3) Se content in vertebrae, liver and whole body was increased with Se level increasing. Se content in vertebrae was extremely significantly affected by Se source ( P<0.01) . Se content in liver and verte⁃brae was extremely significantly affected by the interaction of Se level and Se source ( P<0.01) . In conclusion, using Se⁃Met and Se⁃S as Se sources, the highest SGR of juvenile cobia can be obtained when dietary Se level is 1.29 and 1.46 mg/kg, respectivly, by quadratic polynomial regression equations. With SGR and Se content in whole body as dependent variables, the biological utilization of Se⁃Met is 1.20 and 2.90 times that of Se⁃S, respectivly.关键词
军曹鱼幼鱼/硒/生长性能/抗氧化酶/硒沉积Key words
juvenile cobia ( Rachycentron canadum )/selenium/growth performance/antioxidant enzyme/selenium accumulation分类
农业科技引用本文复制引用
杨原志,聂家全,谭北平,董晓慧,杨奇慧,迟淑艳..硒源与硒水平对军曹鱼幼鱼生长性能、肝脏和血清抗氧化指标及组织硒含量的影响[J].动物营养学报,2016,28(12):174-184,11.基金项目
公益性行业（农业）科研专项（201003020）；广东省农业攻关（2012B020307005）；广东省教育厅引进人才项目 （）
