动物营养学报2016，Vol.28Issue(12)：205-214,10.DOI:10.3969/j.issn.1006-267x.2016.12.026
饲料硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响
Effects of Dietary Selenium Content on Growth Performance, Antioxidant Capacity and Lipid Metabolism Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)
摘要
Abstract
This experiment was conducted to investigate the effects of dietary selenium content on growth per⁃formance, antioxidant capacity and lipid metabolism gene expression of juvenile yellow catfish ( Pelteobagrus fulvidraco). A total of 450 juvenile yellow catfish with the initial body weight of (2.12±0.01) g were random⁃ly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The fish in those groups were fed 6 isonitrogenous and isolipid experimental diets containing <0.05 (control group), 0.15, 0.23, 0.35, 0.47 and 0. 96 mg/kg selenium for 8 weeks, respectively. The results showed as follows: the weight gain rate ( WGR) increased firstly and decreased subsequently with dietary selenium content increasing, and the highest value appeared at the 0. 23 mg/kg selenium group, which was significantly higher than that in control group ( P<0.05) . The feed conversion rate ( FCR) exhibited an opposite trend to the WGR, and the 0.23 mg/kg se⁃lenium group had the lowest value, which was significantly lower than that in control group (P<0.05). The survival ratio in all groups was 100%. The highest whole body crude protein content was found in the 0.35 mg/kg selenium group, which was significantly higher than that in control group and 0.23 mg/kg seleni⁃um group ( P<0.05) . The whole body crude lipid content in control group was significantly lower than that in 0.15, 0.47 and 0.96 mg/kg selenium groups ( P<0.05) , but the whole body moisture content in control group was significantly higher than that in 0.15, 0.35, 0.47 and 0.96 mg/kg selenium groups ( P<0.05) . The plas⁃ma cholesterol ( CHO) content in 0.23 mg/kg selenium group was the lowest, and was significantly lower than that in control group ( P<0.05) . The whole body selenium content and liver glutathione peroxidase ( GPx) ac⁃tivity were enhanced with dietary selenium content increasing, and presented a dose⁃response relationship. The liver superoxide dismutase ( SOD) activity was firstly increased and then decreased with dietary selenium con⁃tent increased from <0. 05 to 0. 47 mg/kg, and then was increased again when dietary selenium content was 0.96 mg/kg. The relative expression levels of lipoprotein lipase ( LPL) and lipoprotein lipase ( FAS) mRNA in control group were significantly lower than those in 0.23 mg/kg selenium group ( P<0.05) . Using WGR as the evaluation index, the suitable dietary selenium content of juvenile yellow catfish is estimated to be 0.20 mg/kg by nonlinear regression analysis.关键词
硒/黄颡鱼幼鱼/生长性能/抗氧化能力/脂肪代谢基因表达Key words
selenium/juvenile yellow catfish/growth performance/antioxidant capacity/lipid metabolism gene expression分类
农业科技引用本文复制引用
胡俊茹,王国霞,孙育平,陈冰,曹俊明,黄燕华,卓丽欣..饲料硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响[J].动物营养学报,2016,28(12):205-214,10.基金项目
2011年广东省农业科技项目 （）
