动物营养学报2016，Vol.28Issue(12)：223-231,9.DOI:10.3969/j.issn.1006-267x.2016.12.028
早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子和小肠黏膜免疫相关细胞的影响
Effects of Early Administration of Ruminal Fluid on Plasma Immunoglobins and Cytokines, and Mucosal Immunity-Associated Cells in Small Intestine in Lambs
摘要
Abstract
This experiment aimed to study the effects of early administration of ruminal fluid on plasma immu⁃noglobins and cytokines, and mucosal immunity⁃associated cells in small intestine in lambs. Twelve Texel new⁃born lambs were randomly allotted to two groups with 6 lambs per group. Ruminal fluid was orally administered once daily over a period of 14 days in trial group, and physiological saline was administered in control group. Blood was collected from jugular vein to separate plasma at 7, 14, 21 and 28 days of age, respectively;lambs were slaughtered to obtain small intestinal digesta at 28 days of age. Immunoglobins [ immunoglobin A ( IgA) and immunoglobin G (IgG)] and cytokines [interleukin⁃1β (IL⁃1β), interleukin⁃6(IL⁃6), tumor necrosis factor α ( TNF⁃α) and interferon γ ( IFN⁃γ) ] contents in plasma, and IgG and immunoglobin Fc fragment ( Fc) contents in intestinal digesta were determined. The results showed as follows:at 21 days of age, the con⁃tents of IgA and IgG in plasma in trial group was significantly lower than those in control group ( P<0.05 or P<0.01) , but there were no significant differences at the other days of ages ( P>0.05);the contents of IL⁃1βand IL⁃6 in plasma in trial group were higher than those in control group, but the differences were not signifi⁃cant ( P>0.05); plasma TNF⁃α content in trial group was significantly higher than that in control group at 14 days of age ( P<0.05) , but there were no significant differences at the other days of ages ( P>0.05); plasma IFN⁃γ content in trial group was higher than that in control group, but the difference was not significant ( P>0.05); there was no significant difference of IgG and Fc contents in intestinal digesta at 28 days of age ( P>0.05); the administration of ruminal fluid increased intestinal intraepithelial lymphocyte count, especially in il⁃eum, trial group was significantly different from control group ( P<0.05);mast cell count at different intestinal segments was not significantly different between groups ( P>0.05) . It is concluded that early administration of ruminal fluid has no influence on humoral immune in lambs, while to some extent, can enhance cell⁃mediated immune; early administration of ruminal fluid has no influence on intestinal transport of maternal immunoglo⁃bins in lambs; early administration of ruminal fluid can stimulate intestinal mucosal immune system in lambs, and contribute to immunologic barrier establishment.关键词
新生羔羊/瘤胃液/血浆/免疫球蛋白/细胞因子/黏膜免疫相关细胞Key words
newborn lamb/ruminal fluid/plasma/immunoglobin/cytokine/mucosal immunity-associated cell分类
农业科技引用本文复制引用
吴婷婷,韩冰,刘振,何周瑞,杨开伦..早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子和小肠黏膜免疫相关细胞的影响[J].动物营养学报,2016,28(12):223-231,9.基金项目
2011国家自然科学基金 （）
