中国妇幼健康研究2016，Vol.27Issue(11)：1353-1355,1356,4.DOI:10.3969/j.issn.1673-5293.2016.11.019
P57免疫组化结合CEP17倍体分析在葡萄胎鉴别诊断中的作用
Application of P57 immunohistochemistry and CEP17 ploidy analysis in differential diagnosis of hydatidiform mole
摘要
Abstract
Objective To investigate the significance of P 57 immunohistochemistry and CEP 17 ploidy analysis in differential diagnosis of hydatidiform mole (HM).Methods The tissues amples, including 45 cases of complete hydatidiform mole (CHM), 25 cases of partial hydatidiform mole (PHM) and 25 cases of hydropic abortuses (HA), were collected from Urumqi Maternal and Child Health Hospital . The P57 expression and CEP17 ploidy analysis were analyzed using immunohistochemistry and fluorescence in situ hybridization FISH techniques , respectively .The significance of combining P 57 immunohistochemistry and CEP 17 ploidy analysis in the differential diagnosis of HM was further explored .Results P57 protein expression in PHM and HA was mainly in villous cytotrophoblast cells and stromal cells , and their rates of positive expression were 100%(25/25).But P57 expression was absent in cytotrophoblast cells and stromal cells in most CHM, and the positive expression rate was 4.4%(2/45), which was statistically different from that in PHM and HA (χ2 =61.9, both P<0.05).FISH analysis showed that CEP17 was diploid of CEP17 in 90.9% HA cases.In PHM cases, 87.0% (20/23) showed triploid of CEP17, and in CHM cases, 97.7%(42/43) showed diploid of CEP17.Statistical analysis showed that the incidence of triploid in PHM cases was significantly higher than in HA cases (χ2 =27.3, P<0.05) and CHM cases (χ2 =49.6, P<0.05).Conclusion The combined analysis of P 57 immunohistochemistry and CEP 17 ploidy plays an important role in the differential diagnosis of CHM , PHM and HA.关键词
葡萄胎/流产/免疫组织化学/CEP17倍体分析Key words
hydatidiform mole ( HM)/abortion/immunohistochemistry/CEP17 ploidy analysis分类
医药卫生引用本文复制引用
陈永峰,薛荣,刘璇,陈云新,沈丹华..P57免疫组化结合CEP17倍体分析在葡萄胎鉴别诊断中的作用[J].中国妇幼健康研究,2016,27(11):1353-1355,1356,4.基金项目
乌鲁木齐市科学技术局基金资助项目 （）
