华西口腔医学杂志2016，Vol.34Issue(6)：579-583,5.DOI:10.7518/hxkq.2016.06.006
Nd:YAG激光对牙根面结构及变异链球菌黏附的影响
Effects of Nd: YAG laser irradiation on the root surfaces and adhesion ofStreptococcus mutans
李圆红 1李忠成 1罗梦奇 1申道南 1张舒 1孟姝1
作者信息
- 1. 口腔疾病研究国家重点实验室华西口腔医院牙周病科(四川大学),成都 610041
- 折叠
摘要
Abstract
Objective This study aimed to evaluate the effects of treatment with different powers of Nd: YAG laser irra-diation on root surfaces andStreptococcus mutans (S. mutans) adhesion.Methods Extracted teeth because of severe perio-dontal disease were divided into the following four groups: control group, laser group 1, laser group 2, and laser group 3. After scaling and root planning, laser group 1, laser group 2, and laser group 3 were separately treated with Nd: YAG laser irradiation (4/6/8 W, 60 s); however, the control group did not receive the treatment. Scanning electron microscopy (SEM) was used to determine the morphology.S. mutans were cultured with root slices from each group. Colony forming unit per mL (CFU·mL-1) was used to count and compare the amounts of bacteria adhesion among groups. SEM was used to observe the difference of bacteria adhesion to root surfaces between control group (scaling) and laser group 2 (6 W, 60 s), thereby indicating the diffe-rent bacteria adhesions because of different treatments.Results Morphology alterations indicated that root surfaces in control group contain obvious smear layer, debris, and biofilm; whereas the root surfaces in laser group contain more cracks with less smear layer and debris. The bacteria counting indicated thatS. mutans adhesion to laser group was weaker than that of control group (P<0.05). No statistical significance among the laser groups (P>0.05) was observed. Morphology alterations also verified thatS. mutans adhesion to laser group 2 (6 W, 60 s) was weaker than that of control group (scaling).Conclusion This study demonstrated that Nd: YAG laser irradiation treatment after scaling can reduce smear layer, debris, and biofilm on the root surfaces as compared with conventional scaling. The laser treatment reduces the adhesion ofS. mutans as well. However, Nd: YAG laser irradiation can cause cracks on the root surfaces. In this experiment, the optimum laser power of 6 W can thoroughly remove the smear layer and debris, as well as relatively improve the control of thermal damagee.关键词
Nd:YAG激光/变异链球菌/根面Key words
Nd: YAG laser/Streptococcus mutans/root surfaces分类
医药卫生引用本文复制引用
李圆红,李忠成,罗梦奇,申道南,张舒,孟姝..Nd:YAG激光对牙根面结构及变异链球菌黏附的影响[J].华西口腔医学杂志,2016,34(6):579-583,5.
