临床口腔医学杂志2016，Vol.32Issue(12)：707-710,4.DOI:10.3969/j.issn.1003-1634.2016.12.002
不同浓度无机磷对MC3 T3-E1细胞系成骨及破骨相关基因表达的影响
The effects of inorganic phosphate at diferent concentrations on osteoblastic and osteoclastic genes of cultured MC3T3-E1 cells in vitro
摘要
Abstract
Objective:To investigate the effects of different concentrations of inorganic phosphate on associated gene of cultured MC3T3-E1 cells. Methods:MC3T3-E1 cells were cultured in vitro. According to the concentrations of inorganic phosphate in culture media,cells were divided into 4 groups,i. e. 0 mM group( control group) ,1 mM group,3 mM group and 5 mM group. The mRNA expression of associated genes in MC3T3-E1 cells were detected using Real-time PCR. Results:Re-al-time PCR showed that osteoblastic genes( ALP,BSP,OC and OSX) were increased in 1 mM and 3 mM group,decreased in 5 mM group. In contrast,osteoblastic inhibitory gene( SOST) and osteoclastic genes( CTSK,TRAP) were increased in 1 mM,3 mM and 5 mM groups. Conclusion:The results indicate that high concentration(5 mM) of inorganic phosphate could inhibit the osteogenic activity of MC3T3-E1 cells by increasing the osteoblastic genes and decreasing the osteoclastic genes.关键词
无机磷/MC3T3-E1细胞系/成骨相关基因/破骨相关基因/基因调节Key words
Inorganic phosphate/MC3T3-E1 cells/Osteoblastic genes/Osteoclastic genes/Gene regulation分类
生物科学引用本文复制引用
郭慧慧,申晋斌,冯娟,张璐,张蓉..不同浓度无机磷对MC3 T3-E1细胞系成骨及破骨相关基因表达的影响[J].临床口腔医学杂志,2016,32(12):707-710,4.基金项目
国家自然科学基金（31170898）回国人员启动基金 （）
