临床口腔医学杂志2016，Vol.32Issue(12)：711-713,714,4.DOI:10.3969/j.issn.1003-1634.2016.12.003
60 Coγ射线照射大鼠建立放射性口腔黏膜炎模型的初步研究
Preliminary research on the establishment of non-invasive model of radiation-induced oral mucositis in rats by 60 Coγ-rays irradiation
摘要
Abstract
Objective:To establish a non-invasive model of radiation-induced oral mucositis( RTOM) in rats and ob-serve the dynamic process of the occurrence,development and healing of mucosal inflammation. Methods:After randomly di-viding 45 SD rats into 9 groups,one group was maintained as a blank control group,while each rat of the other 8 groups re-ceived an irradiation of single 35 Gy dose of 60 Coγ-rays. The condition of the oral mucosal was observed everyday. Each group of experimental rats was sacrificed at the 1 st , 3 rd , 5 th , 7 th , 10 th , 14 th , 21 st and 28 th day after the irradiation respectively. However,the control group of rats were sacrificed only on the 28th day. Oral mucosa tissues of the dead rats were harvested. The samples underwent HE staining for histological observation. Results:Clinically,after 5 to 6 days of irradiation, redness and swelling of oral mucosa along with formation of small ulcers were observed. In the 7th to 8th day of irradiation, small ulcers gradually merged into large ones. From the 14th day of the 35 Gy radiation dose delivery,the ulcers began to un-dergo gradual healing. They were found to be completely healed within 21 st to 28 th days after irradiation. The HE staining showed that a large number of inflammatory cells appeared in the submucosal layer after 5 days of irradiation,but the epitheli-al lamina was observed to be intact. Fragmentation and exfoliation of epithelial layer was observed only at the 7 th to 10 th day of irradiation. New blood capillaries and granulation tissue could be observed at the bottom of the ulceration at the 14 th day of ir-radiation whereas at the 21st and 28th day,the process of re-epithelialization was noted to have started and the basal layer cells were arranged closely. Conclusion:A model of 60 Coγ-rays irradiation induced oral mucositis in rats was established. Inflam-mation was seen to occur at day 5 to 8 of irradiation which significantly reached its peak at day 9 to 13. Lesion gradually be-gan to undergo healing after day 14 and showed full recovery by the day 21 to 28 of irradiation exposure.关键词
放射性/口腔黏膜炎/大鼠Key words
Radioactivity/Oralmucositis/Rat分类
医药卫生引用本文复制引用
刘礼,陶人川,雍翔智,蒋兰岚,曾启新..60 Coγ射线照射大鼠建立放射性口腔黏膜炎模型的初步研究[J].临床口腔医学杂志,2016,32(12):711-713,714,4.基金项目
广西科学基金重点项目 （）
