临床神经外科杂志2016，Vol.13Issue(6)：428-431,4.DOI:10.3969/j.issn.1672-7770.2016.06.008
FRK通过抑制ERK信号通路对脑胶质瘤细胞增殖作用的研究
Study on effect of FRK on proliferation of glioma cells by inhibiting ERK signaling pathway
摘要
Abstract
Objective To study the mechanism of FRK regulating glioma cells proliferation through inhibiting ERK signaling pathway .Methods FRK plasmid was transfected into U 251 cells by PolyJetTM.Western blot(WB) was applied to detect the efficiency of FRK over-expression and P-ERK, ERK protein levels, and EDU incorporation assay was used to explore the effect of FRK over-expression on glioma U251 cells proliferation.After treatment with ERK inhibitor PD98059, the protein level of FRK , P-ERK and ERK was tested by WB , and the proliferation ability of glioma U251 cells was examined by EDU incorporation assay .Results WB results showed that the FRK plasmid was transfected into glioma U 251 cells successfully , FRK over-expression decreased the proliferation ability of glioma U251 cells.And FRK over-expression decreased the protein level of P-ERK, but had no effect on the protein level of ERK . Compared with control group , the phosphorylation of ERK decreased significantly in PD 98059 treatment group , but the protein level of FRK had no change.The proliferation ability of U251 cells was significantly decreased after treatment with ERK inhibitor PD98059.Conclusion FRK may regulate the proliferation of glioma U251 cells via inhibiting ERK pathway .关键词
脑胶质瘤/增殖/FRK/ERKKey words
glioma/proliferation/FRK/ERK引用本文复制引用
金戈,石琼,张道为,王军,蔡畅,宋旭,周秀萍,于如同..FRK通过抑制ERK信号通路对脑胶质瘤细胞增殖作用的研究[J].临床神经外科杂志,2016,13(6):428-431,4.基金项目
国家自然科学基金(基金编号81272777,81372699,81472345,81402046) （基金编号81272777,81372699,81472345,81402046）
