临床输血与检验2016，Vol.18Issue(6)：517-520,4.DOI:10.3969/j.issn.1671-2587.2016.06.002
创伤性失血性休克对机体抗氧化酶系统影响的实验研究
Experimental Study on the Influences of Antioxidant Enzyme Systems of Organism on Traumatic Hemorrhagic shock
摘要
Abstract
Objective To study the influence of traumatic hemorrhagic shock(THS)on antioxidant enzymes. Methods The model of the THS with gradient decrease of the systolic blood pressure was etablished in11 Newzealand rabbits.The arterial blood pressures in the different time following THS were measured with the systolic pressures of2/3 (T2),1/2(T3),and1/3(T4)of the basic systolic blood pressure Superoxide dismutase(SOD),hydrogen peroxide enzyme(CAT),and glutathione oxidation reductase(GSH-Px)levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbent assay(ELISH).Results T-SOD:The levels of the antioxidant enzymes were lower in Group A than in group B; group B was higher than group C; and group C was higher than group D. There was no significant difference between the time level(P>0.05).In CAT detection,it was higher in Group A than in group B,group B was higher than group C,group C was higher than group D,there was no significant difference between the T1-T2,T2-T3,and T3-T4(P>0.05). The significant difference,however,was noted between the other groups(P <0.05). In GSH-Px tests,Group A was found to be higher than group B,group B was higher than group C, group C was higher than group D,there was no significant difference between the T1 and T2(P>0.05),and A significant difference was seen between the other groups(P<0.05).Conclusion When the systolic blood pressure decline curve was used for THS and antioxidant detection in rabbits,THS has a significant affect on the antioxidant enzyme systemshowing a significant decrease of CAT,GSH-Px and SOD,and activity decreased. Blood loss coincides with decrease of CAT and GSH-Px on THS.关键词
创伤性失血性休克/脂质过氧化/抗氧化酶/超氧化物歧化酶/过氧化氢酶/谷胱甘肽过氧化物酶Key words
Traumatic/Hemorrhagic shock/Lipid peroxidation/Antioxidant enzyme/Superoxide dismutase/Hydrogen peroxide enzyme/Glutathione oxidation reductase分类
医药卫生引用本文复制引用
李大鹏,吕春雷,王永清,王凤娇,郑文哲..创伤性失血性休克对机体抗氧化酶系统影响的实验研究[J].临床输血与检验,2016,18(6):517-520,4.基金项目
本课题受军队“十二五”后勤科研计划项目（No.CWS11J297）资助 （）
