临床输血与检验2016，Vol.18Issue(6)：524-527,4.DOI:10.3969/j.issn.1671-2587.2016.06.004
Rh（D）变异体的分子生物学研究
Study on Molecular Biology of Rh(D) Variants
摘要
Abstract
Objective To identify3 RhD variants by polymerase chain reactions(PCRs).Methods Rh blood grouping was carried out by standard serologic test. PCR-SSP was used to amplify theRHD-specific polymorphisms located inRHD promoter,exons,introns,andRHCE-specific polymorphisms.RHD zygosity was determined by PCR-RFLP. Results The serologic results showed that the3 samples carry D variants. PCR-SSP results indicated that these patients harboredRHD*D-CE(3-6)-D hybrid alleles,ie,RHD*DVI.3.RHD zygosity showed the patients withRHD+/RHD-heterozygote.RHCE genotyping indicated that the patients haveRHCE*Ccee. The RH genotyping of these three patients wereCDVI IIIe/cde.Conclusion Genotyping is an efficient method in determining the RhD variants.关键词
Rh血型/RHD基因/Rh(D)变异体/基因分型Key words
Rh blood group/RHD gene/Rh(D) variant/Genotype分类
医药卫生引用本文复制引用
肖建宇,李平,陆乐,王树亚,黄成垠,陈青..Rh（D）变异体的分子生物学研究[J].临床输血与检验,2016,18(6):524-527,4.基金项目
本课题受江苏省重点研发计划社会发展项目（No. BE2015717）、江苏省“十二五”期间“科教兴卫工程”医学重点人才（No.RC2011088）、江苏省第四期“333工程”项目（No.BRA2015501）、江苏省第十批“六大人才高峰”项目（No.2013-WSW-039）、2010~2014年度江苏省卫生国际交流支撑计划项目资助 （）
