临床输血与检验2016，Vol.18Issue(6)：559-561,3.DOI:10.3969/j.issn.1671-2587.2016.06.016
ELISA法不同S/CO值与金标法检测无偿献血者HBsAg相关性研究
Use of ELISA for HBsAg Detection with DifferentS/CO Values and Gold-labeling Method in Volunteer Blood Donors
张毓 1孙国栋 1徐晓艳 1张丽 1王学刚1
作者信息
摘要
Abstract
Objective To Analyze the correlation of HBsAg detection results by ELISA with differentS/CO values and gold-labeling methodfor safe collection of blood and reduction of the waste of blood resources.Methods37966 volunteer blood donors were subjected to detection in Handan area from Nov2014to Apr2015. After primary screening of HBsAg,gold-labeling method was used for re-detection of the samples that showed positive HBsAg in the previous detection by ELISA. The results of ELISA test were compared with those of gold-labeling.Results From Nov2014 to Jan 2015,HBsAg was found to be positive in102 cases of volunteer blood donors,with a positive rate of0.52%. The gold-labeling detection rate seemed to be various in companies(χ2=33.33,P<0.01;χ2=25.47,P<0.01). The false positivity was noted when re-tested by gold-labeling in all of the apecimens below theS/CO value of20,Hereafter,the HBsAg- positive rate showed a gradual reduction(χ2=14.58,P<0.05).Conclusion It is an efficient way for reduction of false positivity of HBsAg to improve the gold-labeling method and operation skills of technicians.关键词
无偿献血者/S/CO值/乙肝表面抗原/相关性研究Key words
Volunteer blood donors/S/CO value/HBsAg/Relevant research分类
医药卫生引用本文复制引用
张毓,孙国栋,徐晓艳,张丽,王学刚..ELISA法不同S/CO值与金标法检测无偿献血者HBsAg相关性研究[J].临床输血与检验,2016,18(6):559-561,3.
