林业科学2016，Vol.52Issue(11)：47-54,8.DOI:10.11707/j.1001-7488.20161106
金属纳米颗粒CCCSNs在植物体内的积累和分布
Accumulation and Allocation of Copper-Carbon Core-Shell Nanoparticles in Three Species
摘要
Abstract
Objective]CCCSNs is a new renewable composite nano-material that is called as the Copper-Carbon Core-Shell Nanoparticles, and it is fabricated by using cotton fibers as the template, with copper atoms homogeneously embedded in carbon black as a composite material. The special carbon shells can protect the copper core from oxidation, by which the Cu shows the typical antibacterial,antifungal,antifouling properties. This paper is expected to explore the the accumulation and dispersion of CCCSNs in Phyllostachys edulis,Dracaena sanderiana and Gossypium hirsutum,in order to provide the basis for the plants to make use of CCCSNs in promoting the anti-corrosion ability.[Method]The P. edulis and D. sanderiana were grown in hydroponic media with adding 0,0. 1 or 0. 5 g L -1 CCCSNs. G. hirsutum was grown in potting soil amended with 0,0. 1 or 0. 5 g CCCSNs per a pot,and watered with tap water. The three plant species were managed regularly. After 50 days,we harvested all samples that were used to test the copper concentration which could be considered as CCCSNs signal. The samples were dissolved by hydrogen nitrate and the extract was measured with atomic absorption spectrum. The anatomy of roots was observed by TEM and DES.[Results]CCCSNs was able to enter a plant and transport from roots to stems and leaves,and the accumulation of CCCSNs was dependent on the different species. The Cu concentration of P. edulis stems and leaves,when their roots exposed to 0. 1 and 0. 5 g·L -1 CCCSNs,was 13. 19 and 11. 79 μg·g -1 ,and were 263% and 225% higher than that of the control,respectively. With 0. 1 and 0. 5 g·L -1 CCCSNs treatment,the copper concentration of D. sanderiana stems and leaves was 29. 31 and 27. 95μg·g -1 and 104% and 90% higher than that of the control,respectively. The Cu concentration in stems and leaves of G. hirsutum treated with 0. 1 and 0. 5 g·pot -1 was 5. 22 and 6. 53 μg·g -1 and 24% and 52% higher than that of the control. The highest Cu concentration in stems and leaves of P. edulis,D. sanderiana and G. hirsutum was 21. 65,44. 88 and 9. 19 μg·g -1 ,respectively. D. sanderiana had the highest average Cu concentration and highest Cu concentration among the three species. However,P. edulis had the highest increasing rate of copper among the three species. Confirmed with TEM and DES detection,CCCSNs was able to accumulate in the cytoplasm,cell membrane,cell wall and intercellular space of root cells. Moreover,there was no difference in CCCSNs distribution pattern in the root cells over the three species. [Conclusion]When adding CCCSNs into the culture media of P. edulis,D. sanderiana and G. hirsutum, CCCSNs could enter the stems and leaves from the roots. The transportation capacity was dependent on the species. The Cu concentration in the plant was positively correlated with quantity of CCCSNs when the added CCCSNs was at a low level. Meanwhile,CCCSNs was able to accumulate in the cytoplasm,cell membrane,cell wall and intercellular space of root cells. Although the experiment had proved that CCCSNs could enter plants from roots,how CCCSNs enters the cells from intercellular space and in which way enters stems or other issues still need further research.关键词
金属纳米颗粒 CCCSNs/积累/分布/毛竹/富贵竹/陆地棉Key words
metal nanoparticles CCCSNs/accumulation/allocation/Phyllostachys edulis/Dracaena sanderiana/Gossypium hirsutum分类
农业科技引用本文复制引用
王安可,毕毓芳,王玉魁,蔡函江,翟志忠,钟浩,杜旭华,丁兴萃,田新立..金属纳米颗粒CCCSNs在植物体内的积累和分布[J].林业科学,2016,52(11):47-54,8.基金项目
国家林业局948项目(2013－4－23) （2013－4－23）
浙江省科技计划项目(2014F10047)。 （2014F10047）
