农药学学报2016，Vol.18Issue(6)：710-716,7.DOI:10.16801/j.issn.1008-7303.2016.0099
环氧虫啶对苜蓿蚜的毒力及对其体内解毒酶活性的影响
Toxicity of cycloxaprid toAphis craccivora (Koch) and its effects on detoxification enzymes
摘要
Abstract
To investigate the effects of bridged-neonicotinoid compounds onAphis craccivora (Koch), the toxicity of cycloxaprid and the other three bridged-neonicotinoid compounds onA. craccivora and its effects on detoxification enzymes were determined by leaf dipping method using imidacloprid as the control. The results showed that cycloxaprid and other seven-membered bridged neonicotinoid compounds displayed high insecticidal activities againstA. craccivora. The LC50 of cycloxaprid was 3.454 mg/L, which is higher than that of the eight-membered bridged neonicotinoid compounds. The synergistic ratios of enzyme inhibitors DEM and PBO were 4.02 and 3.22, respectively. Both compounds showed remarkable synergistic effects on the toxicity of cycloxaprid, whereas only PBO could significantly enhance the toxicity of imidacloprid. Further enzyme activity tests revealed that the activities of detoxification enzymes, namely, glutathioneS-transferase (GSTs) and cytochrome P450 enzymes (7-ethoxycoumarin-O-deethylase) inA. craccivora, could be enhanced significantly (P< 0.05) by the treatment of cycloxaprid and imidacloprid (LC50 level). Although cycloxaprid could enhance the activities of the above two enzymes to (2.730 ± 0.012) and (0.239 ± 0.009) μmol/(mg pro.·min), respectively, it is still lower than that of imidacloprid. No significant change was observed for the activities of carboxylesterase (CarE). The above results indicated that GSTs and P450s might play key roles during the detoxification of cyclcxaprid inA. craccivora.关键词
新烟碱类杀虫剂/环氧虫啶/苜蓿蚜/解毒酶/谷胱甘肽S-转移酶/细胞色素 P450酶Key words
neonicotinoids/cycloxaprid/Aphis craccivora/detoxification enzymes/glutathioneS-transferase/P450 enzyme分类
农业科技引用本文复制引用
吴勇超,须志平,邵旭升,程家高,李忠..环氧虫啶对苜蓿蚜的毒力及对其体内解毒酶活性的影响[J].农药学学报,2016,18(6):710-716,7.基金项目
国家自然科学基金(31501674；21572059) （31501674；21572059）
