山东医药2016，Vol.56Issue(45)：6-8,3.DOI:10.3969/j.issn.1002-266X.2016.45.002
PI3K／Akt 信号传导通路在乙醛刺激下大鼠肝星状细胞株 HSC-T6增殖中的作用
Effects of PI3K/Akt signaling pathway on acetaldehyde-stimulated proliferation of hepatic stellate cells HSC-T6
摘要
Abstract
Objective To investigate the effects of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway spe-cific inhibitor LY294002 on the proliferation of rat hepatic stellate cells (HSC-T6)stimulated by acetaldehyde.Methods The rat HSC-T6 cells were randomly divided into six groups:the blank control group (group A):we used DMEMculture solution with 10% fetal bovine serum (FBS)to culture,the acetaldehyde group (group B):we added acetaldehyde on the basis of the blank control group and made the final concentration be 200 μmol/L,the experimental group was added with different concentrations of PI3K/Akt pathway specific inhibitor LY294002 (25,50,75 and 100 μmol/L)on the basis of acetaldehyde group (group B),and then they were named groups C,D,E and F,respectively.CCK-8 was applied to de-tect the proliferation activity of rat HSC-T6 cells.And then HSC-T6 cells were randomly divided into three groups:the blank control group (added with DMEMculture solution with 10% fetal bovine serum),acetaldehyde group (added with acetaldehyde on the basis of blank control group and made the final concentration be 200 μmol/L),and acetaldehyde +LY294002 group (added with 50 μmol/L PI3K/Akt pathway specific inhibitor LY294002 on the basis of acetaldehyde group).Western blotting was used to detect the expression of PI3K and p-Akt protein in rat HSC-T6.Results The A val-ues of groups A,B,C,D,E,and F were 0.545 0 ±0.024 4,0.952 3 ±0.045 5,0.927 3 ±0.041 9,0.865 8 ±0.028 9,0.814 3 ±0.024 7 and 0.759 3 ±0.220 0,respectively.Compared with group A ,the A values of other groups all in-creased;compared with groups B and C,the A values of groups D,E and F all decreased (all P <0.01).Compared with the blank control group,the protein expression of PI3K and p-Akt in acetaldehyde group and acetaldehyde +LY294002 group were increased (all P <0.01).Compared with the acetaldehyde group,the value of acetaldehyde +LY294002 group decreased (P <0.05).Conclusion PI3K/Akt signaling pathway may partly adjust and control the proliferation of rat HSC-T6 stimulated by acetaldehyde.关键词
肝纤维化/肝星状细胞/乙醛/磷脂酰肌醇 3 激酶/蛋白激酶 B 信号传导通路/LY294002Key words
hepatic fibrosis/hepatic stellate cell/acetaldehyde/phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway/LY294002分类
医药卫生引用本文复制引用
张迪,程敏,康馨丹,陈卫刚,郑勇..PI3K／Akt 信号传导通路在乙醛刺激下大鼠肝星状细胞株 HSC-T6增殖中的作用[J].山东医药,2016,56(45):6-8,3.基金项目
国家自然科学基金资助项目（81170402）。 （）
