山东医药2016，Vol.56Issue(45)：23-26,4.DOI:10.3969/j.issn.1002-266X.2016.45.007
2，3-吲哚醌对神经母细胞瘤细胞 SH-SY5Y侵袭转移的影响及机制
Effect of 2,3-isatin on invasion and metastasis of neuroblastoma SH-SY5Y cells and its mechanism
摘要
Abstract
Objective To investigate the effect of 2,3-isatin on the invasion and metastasis of neuroblastoma SH-SY5Y cells and the mechanism.Methods SH-SY5Y cells cultured in vitro were treated for 48 h with different concentra-tions of 2,3-isatin (25,50,100,200 and 300 μmol/L).The cell viability was measured by CCK-8.The number of cells with autolysosome in the control group and 200 μmol/L isatin-treated group was detected by acridine orange staining fluo-rescence.The expression of LC3 Ⅱ,a marker protein for autophagy was examined by Western blotting.The invasion of SH-SY5Y cells was tested by Transwell assay and the motile ability was detected by Scratch test.Results Compared with the control group,the A450 value decreased and the inhibition rate increased when the concentration of 2,3-isatin was higher than 100 μmol/L (P <0.05 or P <0.01).AO staining showed that the number of autolysosomes increased in the 200 μmol/L 2,3-isatin treatment group as compared with that of the control group (P <0.01).Western blotting showed that the expression of LC3-Ⅱincreased with the increasing drug concentration (P <0.05).Transwell assay and Scratch test showed that the invasion and motile abilities of SH-SY5Y cells diminished with the increasing concentration of 2,3-isatin (all P <0.05).Conclusion 2,3-isatin may inhibit the invasion and metastasis of SH-SY5Y cells by inducing the auto-phagy.关键词
神经母细胞瘤/SH-SY5Y 细胞/2,3-吲哚醌/侵袭/转移Key words
neuroblastoma/SH-SY5Y cells/2,3-isatin/invasion/metastasis分类
医药卫生引用本文复制引用
魏尧悦,侯琳..2，3-吲哚醌对神经母细胞瘤细胞 SH-SY5Y侵袭转移的影响及机制[J].山东医药,2016,56(45):23-26,4.基金项目
国家自然科学基金项目（81472542）。 （）
