山东医药2016，Vol.56Issue(45)：27-30,4.DOI:10.3969/j.issn.1002-266X.2016.45.008
系统性红斑狼疮患者血清 miR-155水平变化及意义
Changes in Ievels of serum miR-155 in patients with systemic lupus erythematosus
摘要
Abstract
Objective To investigate the changes in levels of serum miR-155 in patients with systemic lupus erythe-matosus (SLE)and its significance.Methods Serum samples were collected from 50 patients with SLE (SLE group), and 40 healthy individuals (control group)and 45 SLE patients receiving glucocorticoids treatment (treatment group)were also selected.The serum level of miR-155 was detected by RT-qPCR.Then,the association between serum miR-155 level and clinical characteristics,treatment response of glucocorticoids was analyzed.Moreover,the diagnostic and prognostic value of serum miR-155 in SLE patients receiving glucocorticoid treatment was also investigated.Results In the SLE group,the serum miR-155 level was positively correlated with GFR (r =0.576,P <0.001)and hs-CRP (r =0.406,P =0.003)levels,while was negatively correlated with proteinuria (r =-0.480,P =0.004)and SLEDAI (r =-0.553,P<0.001).However,in the control group,no significant correlation was found between miR-155 and those four indexes. Receiver operator curve analysis showed that the area under the curve (AUC )of miR-155 was 0.867,which was signifi-cantly higher than C4 and hs-CRP (z =3.837,P <0.01 ).Besides,the sensitivity and specificity of miR-155 reached 84% and 78%,respectively.Moreover,the AUC of miR-155 in differentiating response to glucocorticoids was 0.782,with the sensitivity and specificity of 78% and 72%,respectively.The expression of miR-155 was significantly increased in pa-tients showing better response to glucocorticoids treatment (the SLEDAI decreased more than 3 points after treatment)as compared with that of patients showing poor response (the SLEDAI decreased no more than 3 points after treatment)(P <0.05).Then we compared the expression variety of miR-155 in SLE patients before and after the glucocorticoids treatment. Our results showed that a statistical significance was found in patients with better response (P <0.05),while no statistical significance was found in patients with poor response (P >0.05).The proportion of patients showing better response to glu-cocorticoid was significantly higher in the high miR-155 expression group than in the low expression group (χ2 =2.29,P <0.05).More importantly,SLE patients with high miR-155 expression showed longer disease-free survival as compared with that of patients with low miR-155 expression (46 months vs.29 months,P =0.002).Conclusion Serum miR-155 is highly expressed in patients with SLE,and can serve as a potential diagnostic marker,therapeutic target and prognostic in-dicator in SLE.关键词
系统性红斑狼疮/微小 RNA-155/糖皮质激素Key words
systemic lupus erythematosus/microRNA-155/glucocorticoids分类
医药卫生引用本文复制引用
黄阳,葛晓松,吴平..系统性红斑狼疮患者血清 miR-155水平变化及意义[J].山东医药,2016,56(45):27-30,4.基金项目
国家自然科学基金青年项目（81502042）；江苏省自然科学基金青年项目（BK20140171）。 （）
