生物加工过程2016，Vol.14Issue(6)：23-28,40,7.DOI:10.3969/j.issn.1672-3678.2016.06.005
基于PCR RFLP技术鉴定常见食源性致病菌
Identification of common food-borne pathogens based on PCR-RFLP techniques
摘要
Abstract
This study adopted a polymerase chain reaction⁃based restriction fragment length polymorphism (PCR⁃RFLP) method to analyze food⁃borne pathogens mitochondrial.16S rRNA from 19 kinds food⁃borne pathogens were amplified. With ribosomal gene 16S rRNA as the special attractant, a trail to digest the target gene by restrictive enzymes Eco52 Ⅰ, Hind Ⅲ, Mbo Ⅰ, Mlu Ⅰ, Pst Ⅰ, Sal Ⅰ and Xba Ⅰ was carried out after PCR amplification of a specific 400 bp region of this gene,and the restriction fragments were analyzed by agarose gel electrophoresis. The digested products of the 16S rRNA of different food⁃borne pathogens manifested in specific patterns. The lowest detection limit is 1 pg/μL. These results showed that the genotype verification of 16S rRNAs by PCR⁃RFLP method proved to be a good tool for detecting foodborne pathogens.关键词
食源性致病菌/分子标记物/PCR RFLP/细菌分类鉴定Key words
food-borne pathogens/molecule marker/polymerase chain reaction-based restriction fragment length polymorphism(PCR-RFLP)/classification分类
农业科技引用本文复制引用
霍胜楠,董海龙,郑世超,孟静,杨振东..基于PCR RFLP技术鉴定常见食源性致病菌[J].生物加工过程,2016,14(6):23-28,40,7.基金项目
山东省优秀中青年科学家科研奖励基金 （）
