生物加工过程2016，Vol.14Issue(6)：54-60,7.DOI:10.3969/j.issn.1672-3678.2016.06.010
解淀粉芽孢杆菌产凝乳酶发酵优化
Optimizing of medium and fermentation conditions for milk-clotting enzyme produced by Bacillus amyloliquefaciens
摘要
Abstract
We optimized of culture medium and fermentation conditions to produce milk⁃clotting enzyme produced by Bacillus amyloliquefaciens JNU002. The results of single factor experiments showed that the best carbon source was lactose;nitrogen source had no obvious promoting effect;KH2PO4 and CaCO3 had promoting effect on enzyme production.Through the orthogonal experiment, the optimum culture medium composition was obtained: bran 16 g/L,lactose 10 g/L,KH2 PO4 1 g/L,CaCO3 1 g/L. Bran had great influence on milk⁃clotting enzyme production.The culture temperature was 37℃,incubation time of liquid seed was 18 h,inoculum amount was 0�5%,the liquid volume 100 mL/500 mL.In the optimized medium and under the optimized culture condition, milk⁃clotting enzyme activity of Bacillus amyloliquefaciens JNU002 reached 12 766 SU/mL an increase of 3�5 times.关键词
解淀粉芽孢杆菌/凝乳酶/优化Key words
Bacillus amyloliquefaciens/milk-clotting enzyme/optimization分类
化学化工引用本文复制引用
王楠,苑畅,汪瑞,淳于彦洁,吴雅雯,李金宝,丁重阳..解淀粉芽孢杆菌产凝乳酶发酵优化[J].生物加工过程,2016,14(6):54-60,7.基金项目
“十二五”国家科技支撑计划（2013BAD18B02）；国家星火计划重点项目（2015GA690004）；江苏省重点研发计划（现代农业）项目（ BE2015306）；江苏省产学研合作项目 （）
