生物加工过程2016，Vol.14Issue(6)：66-70,5.DOI:10.3969/j.issn.1672-3678.2016.06.012
高效液相色谱法测定茶鲜叶中游离多胺含量
Determination of free polyamines in fresh tea leaves by high performance liquid chromatography
摘要
Abstract
We determined putrescine ( Put) ,spermidine ( Spd) and spermine ( Spm) in fresh tea leaves by high performance liquid chromatography.The samples were extracted with 5% ( V/V) perchloric acid (HClO4) containing 67 g/L PVPP (w/V),and then the three free⁃polyamines were derivatized with benzoyl chloride as derivatization agent�The polyamine derivatives were separated on a C18 column by HPLC with ultrapure water and methanol as mobile phases at a flow rate of 1�0 mL/min�The linearity was excellent in the range between 5 and 60 nmol/L with the correlation coefficients above 0�980�The limits of detection( S/N=3) were between 0�11 and 0�41 nmol/L,and the limits of quantification ( S/N=10) were between 0�55 and 2�05 nmol/L�The recovery of standard addition was between 88�5% and 105�5% with the relative standard deviations ( n=3) between 0�99%and 7�20%�Based on this method, the 3 free polyamines were checked out in bud,leaf and stem of fresh tea leaves.The results indicated that the method is sensitive,efficient and accurate�It is suitable for the simultaneous determination of the 3 free polyamines in fresh tea leaves.关键词
茶鲜叶/腐胺/亚精胺/精胺/高效液相色谱Key words
fresh tea leaves/putrescine ( Put)/spermidine ( Spd)/spermine ( Spm)/high performance liquid chromatography ( HPLC)分类
化学化工引用本文复制引用
吴琴燕,陈露,张文文,马圣洲,赵飞,庄义庆..高效液相色谱法测定茶鲜叶中游离多胺含量[J].生物加工过程,2016,14(6):66-70,5.基金项目
江苏省农业科技自主创新资金（CX（14）2122） （）
