现代检验医学杂志2016，Vol.31Issue(6)：10-12,16,4.DOI:10.3969/j.issn.1671-7414.2016.06.003
miR-205在ITP患者外周血单个核细胞中的表达及意义
Expression of miR-2 0 5 in the Peripheral Blood Mononuclear Cells from Patients with Primary Immune Thrombocytopenia
摘要
Abstract
Objective To investigate the expression and clinical significance of microRNA (miR-205)in the peripheral blood mononuclear cells (PBMCs)of Primary Immune Thrombocytopenia (ITP)patients.Methods The real-time fluorescent quantitative PCR (real time PCR)was used to determine the expression of miR-205 in the peripheral blood of 43 ITP pa-tients and 38 healthy controls.The correlations between the expression of miR-205 and platelet counts was analysed.Results The relative expression levels of miR-205 in health controls (0.92±0.25)were significantly lower than that in naive ITP (1.81±0.48)and incmplete remission group (1.63±0.65)(all P<0.01).The difference of miR-205 level between incm-plete remission group and naive ITP group did not reach statistical significance (P=0.112).Expression of miR-205 after complete remission in peripheral blood mononuclear cells (PBMC)was lower than its level at diagnosis expression of miR-205 (1.07±0.43 vs 1.91±0.34,t=5.68,P<0.01).In addition.the miR-205 level in ITP patients was negativel correlated with the platelet counts (r=-0.0696,P<0.05).Conclusion The miR-205 expression in ITP patients was significantly in-creased,indicating miR-205 play an important role in the pathogenes of ITP,may becom a new potential diagnosis and treat-ment target of ITP.关键词
血小板减少/外周血单个核细胞/miR-205/血小板计数Key words
hrombocytopenia/peripheral blood mononuclear cells/miR-205/platelet count分类
医药卫生引用本文复制引用
邓顺江,邓安梅,黄韦华,吴林洪,张蕾,叶辛,刘鹏,李腾达,龙曙萍,钱琤..miR-205在ITP患者外周血单个核细胞中的表达及意义[J].现代检验医学杂志,2016,31(6):10-12,16,4.基金项目
973计划（2013CB531606），国家自然科学基金（81471605，81401358，81501397，31500721，81501398，81302579，81273282，81202353），上海申康基金（SHDC22014014），上海科学教育基金（D14017），军队科研基金（BWS14J023，12MA056，5ZD009，15XD007），美捷登基金（MJR20150019）。 （）
