现代检验医学杂志2016，Vol.31Issue(6)：17-19,3.DOI:10.3969/j.issn.1671-7414.2016.06.005
miR-491-5 p在原发免疫性血小板减少症患者中的表达及临床意义
Expression and Clinical Significance of miR-491-5p in Patients with Primary Immune Tombocytopenia
摘要
Abstract
Objective To explore the expression and clinical significance of microRNA (miR-491-5p)in the peripheral blood mononuclear cells (PBMCs)of primary Immune Thrombocytopenia (ITP)patients.Methods 40 ITP patients (study group)and 40 healthy subjects (control group)were collected from the Department of Hematology,100th Hospital of PLA Hospital.After treatment,22 cases of complete remission as a complete remission.2ml peripheral blood taken from them, PBMCs were separated and total RNA was extracted from the cells,cDNA was synthesized by reverse transcription,U6 for reference,then determined the expression of miR-491-5p with the real-time fluorescent quantitative PCR (real time PCR);To analyze the correlation between miR-491-5P and platelet count.Results Compared newly diagnosed group (5.07±1.70) with control group (1.92±0.95)expression in peripheral blood mononuclear cells up-regulated miR-491-5P compared,the difference was statistically significant (t=9.36,P<0.01).Compared complete remission group (2.32±1.07)with their first visit (4.95±0.83)the expression of miR-491-5P in peripheral blood mononuclear cells compared to cut,the difference was statistically significant (t=8.94,P<0.01).The miR-491-5P level in ITP patients was negativel correlated with the platelet counts (r=-0.769 7,P<0.05).Conclusion The miR-491-5P expression in ITP patients was significantly in-creased,which suggests that it may be related to the pathogenesis of ITP,or to be a potential marker for the diagnosis and treatment of ITP in the future.关键词
原发免疫性血小板减少症/miR-491-5P/血小板计数/外周血单个核细胞Key words
primary immune thrombocytopenia/miR-491-5P/platelet count/peripheral blood mononuclear cells分类
医药卫生引用本文复制引用
丁磊,刘鹏,叶辛,邓安梅,任永亚,吴天勤,钱琤..miR-491-5 p在原发免疫性血小板减少症患者中的表达及临床意义[J].现代检验医学杂志,2016,31(6):17-19,3.基金项目
973计划（2013CB531606），国家自然科学基金（81471605，81401358，81501397，31500721，81501398，81302579，81273282，81202353），上海申康基金（SHDC22014014），上海科学教育基金（D14017），军队科研基金（BWS14J023，12MA056，15ZD009），美捷登基金（MJR20150019）。 （）
