现代检验医学杂志2016，Vol.31Issue(6)：44-47,4.DOI:10.3969/j.issn.1671-7414.2016.06.012
血清miRNA-186，miRNA-30 c在肝细胞性癌中诊断价值的研究
Research of Diagnostic Application of Serum miRNA-1 8 6 and miRNA-30c in Patients with Hepatocellular Carcinoma
摘要
Abstract
Objective To explore the diagnostic value of miRNA-186 and miRNA-30c in patients with hepatocellular carcino-ma(HCC).Methods Gene expression of miRNA-186 and miRNA-30c were examined in 55 serum samples from hepatocel-lular carcinoma(HCC),liver cirrhosis(LC)and health volunteers by quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR).The diagnostic efficiency of miRNA-186 and miRNA-30c on HCC were analyzed by receiver op-erating characteristiccurve (ROC)and compared with alpha-fetoprotein(AFP).Results Expression levels of miR-186 in HCC sera were significant higher than those in other groups(P<0.01 and P=0.02)and miR-30c in HCC sera were signifi-cant lower than those in other groups(P=0.034 and P<0.01).The areas under ROC of miRNA-186 and miRNA-30c in se-ra were 0.927 and 0.932 respectively.miRNA-186 had a sensitivity of 78.18% and a specificity of 63.64% and miR-30c had a sensitivity of 81.82% and a specificity of 71.82% in discriminating HCC from CHB,respectively.The HCC diagnostic sensitivity was up to 89.09%,85.45%,90.9% and 94.54% with the specificity of 66.36%,67.27%,82.73% and 53.64%in the combination of miR-186+miR-30c,miR-186+AFP,miR-30c+AFP and miR-186+miR-30c+AFP respectively.Con-clusion Combination of miR-30c and AFP improved the sensitivity while maintain a relative high specificity,so this combi-nation is worth to be used in clinical practice.关键词
肝癌/miR-186/miR-30c/甲胎蛋白/诊断Key words
hepatocellular carcinoma/miR-186/miR-30c/AFP/diagnosis分类
医药卫生引用本文复制引用
刘冲,唐浩,邓霖,张静,邓建平..血清miRNA-186，miRNA-30 c在肝细胞性癌中诊断价值的研究[J].现代检验医学杂志,2016,31(6):44-47,4.基金项目
湖北省黄石市科技攻关项目，项目编号HWJ2016-11-057。 （）
