现代检验医学杂志2016，Vol.31Issue(6)：66-69,73,5.DOI:10.3969/j.issn.1671-7414.2016.06.018
马钱子碱对人慢性粒细胞白血病KCL-22细胞的增殖及凋亡作用
Effects of Brucine on Proliferation and Apoptosis of Chronic Myelogenous Leukemia KCL-2 2 Cell Line
摘要
Abstract
Objective To study the inhibition and apoptosis of brucine on chronic myelogenous leukemia KCL-22 cells,and then provide a new direction for the treatment of CML.Methods Different concentrations of brucine was added into the KCL-22 cell line in vitro.The inhibition effect of brucine on growth of KCL-22 cells was measured by MTS method.FITC-Annexin-V/PI double labeling method was used to assay the apoptosis rate of KCL-22 after different concentration of bru-cine treatment.The expression of apoptosis-related protein was assayed with Western blotting.Results Brucine displayed significant anti-proliferative effect on KCL-22 cells in a dose-and time-dependent manner.Brucine could significantly induced KCL-22 cell apoptosis in the concentration range from 50μg/ml to 400μg/ml,mainly for early apoptosis.Western blotting showed that the expression of Bcl-2 was reduced,the expression of Bax and Cyt-C were increased,and the Caspase-9, Caspase-3 were activated.Conclusion Brucine could inhibits the proliferation of KCL-22 cells,and induce apoptosis possibly though endogenous mitochondrial pathway by regulating the Bax/Bcl-2 balance,releasing Cyt-C and activating Caspase-9 and Caspase-3.关键词
马钱子碱/慢性粒细胞白血病/KCL-22细胞/增殖抑制/凋亡Key words
brucine/chronic myelogenous leukemia/KCL-22 cell line/proliferation inhibition/apoptosis分类
医药卫生引用本文复制引用
韩泽平,谢杏仪,何金花,黎毓光,吕钰冰,周嘉彬..马钱子碱对人慢性粒细胞白血病KCL-22细胞的增殖及凋亡作用[J].现代检验医学杂志,2016,31(6):66-69,73,5.基金项目
广东省建设中医药强省项目（20141230）；广州市番禺区科技和信息化局项目（2014-Z03-65）。 （）
