现代妇产科进展2016，Vol.25Issue(12)：891-894,899,5.DOI:10.13283/j.cnki.xdfckjz.2016.12.003
监测外周血SCF对预测c-kit阳性上皮性卵巢癌化疗耐药的临床价值
Clinical value of peripheral blood SCF in the prediction of chemoresistance of c-kit posi-tive ovarian cancer
彭光彩 1王晓雯 1易村犍1
作者信息
- 1. 长江大学附属第一医院妇产科，荆州 434300
- 折叠
摘要
Abstract
Objective:To monitor the changes in the concentration of free stem cell fac-tor ( SCF) in peripheral blood and the expression of c-kit protein in ovarian cancer tissues,and to investigate the clinical value of early prediction of c-kit (+) in epithelial ovarian cancer (EOC) resistance to platinum-based chemotherapy by monitoring SCF in peripheral blood. Methods:Expressions of c-kit and SCF were retrospectively studied with quantum dots immuno-fluorescence histochemistry ( QDs-IHC) ,in paraffin-embeded tissue specimens from 106 cases of EOC. The plasma concentration of the SCF were measured with enzyme linked immuno-sor-bent assay ( ELISA) from 46 EOC. To investigate the difference of SCF value in peripheral ser-um and c-kit expression of EOC patients who were sensitive and resistant to platinum-based chemotherapy. Results:In epithelial ovarian cancer ( EOC) ,the positive expression rate of c-kit in the chemoresistance group was 29. 27%,it was statistically higher than chemosensitive group (10. 8%)(P<0. 05). The positive expression of c-kit in EOC was closely corralated with ad-vanced FIGO clinical stages and chemotherapy-sensitivity. The positive rate increased in ad-vanced stage (Ⅰ~Ⅱ8 . 7%,Ⅲ~Ⅳ25%) ( P<0 . 05 ) . The mean plasma concentration of the SCF in the chemoresistance group was statistically higher than the chemosensitive group of EOC ( P<0 . 05 ) . The plasma concentration of the SCF in the chemoresistance group was up-regula-ted after platinum-based chemotherapy. The mean value of the SCF in c-kit (+) group was higher than that in c-kit (-) group in epithelial ovarian cancer(P=0. 018),the plasma con-centration of the SCF in c-kit (+) group was up-regulated after platinum-based chemotherapy. Conclusion:The abnormal expression of c-kit protein may play an important role in the progres-sion and chemoresistance of EOC. The positive expression of c-kit protein in ovarian cancer stromal cell indicated poor clinical outcome. Monitoring of SCF in peripheral blood during plati-num-based chemotherapy, the elevation of serum SCF may be one of the early prediction of chemotherapy resistance in the c-kit(+) EOC.关键词
卵巢上皮性癌/化疗耐药/化疗敏感性/c-kit/SCFKey words
Epithelial ovarian cancer ( EOC )/Chemoresistance/Chemosensitive/c-kit/SCF分类
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彭光彩,王晓雯,易村犍..监测外周血SCF对预测c-kit阳性上皮性卵巢癌化疗耐药的临床价值[J].现代妇产科进展,2016,25(12):891-894,899,5.
