新疆农业科学2016,Vol.53Issue(11):1961-1968,8.DOI:10.6048/j.issn.1001-4330.2016.11.001
粳稻种质资源SSR指纹图谱构建及遗传多样性分析
DNA Fingerprints and Genetic Diversity Analysis Based on SSR Markers for Japonica Rice Resources in Xinjiang
摘要
Abstract
Objective]The study aims to establish the DNA fingerprints and throw light on the genetic diversity of the Japonica Rice in Xinjiang.[Method]The Japonica Rice of 89 varieties were used as materials to analyze the genetic diversity and genetic relationship by 70 SSR markers.[Result]The result showed that there were 63 pairs of primers with polymorphic fragments and 388 effective alleles were detected.Namely, each pair had 6.158,7.The average polymorphism frequency index was 0.237,1 and the genetic similarity coefficient between the 89 materials was 0.72~0.88 with high similarity.If 0.73 was taken as a standard,all materials could be divided into 5 groups,but most were in one group.[Conclusion]However,generally speaking,the genetic background comparability was high and the diversity was low.It is demonstrated that the genetic basis of the Japonica Rice in Xinjiang is narrow.Therefore,identifying and making use of the new ge-netic resources and creating breeding materials should be strengthened.关键词
粳稻/种质资源/遗传多样性Key words
japonica rice/germplasm resources/genetic diversity分类
农业科技引用本文复制引用
张燕红,王奉斌,布哈丽且木·阿不力孜,袁杰,赵志强,孝荣,唐福森,马盾..粳稻种质资源SSR指纹图谱构建及遗传多样性分析[J].新疆农业科学,2016,53(11):1961-1968,8.基金项目
国家科技支撑计划课题(2014BAA03B04);新疆农业科学院青年科技基金项目(xjnkq-2014029)@@@@Supported by national science and technology support projects (2014BAA03B04)and the youth funds of Xinjiang Academy of Agri-cultural Sciences "Analysis of genetic diversity of upland rice germplasm resources in Xinjiang based on SSR markers" ()