新疆农业科学2016，Vol.53Issue(11)：1980-1987,8.DOI:10.6048/j.issn.1001-4330.2016.11.003
海岛棉抗枯萎病相关基因实时荧光定量PCR分析
A Real-time PCR of Related Marker Gene in Cotton (Gossypium barbadense L.)
摘要
Abstract
Objective]With different resistant island cottons as material,using real-time fluorescence quantitative PCR technology to analyze the expression levels of 1 1 genes related to wilt resistance in cotton, this project aims to provide candidate gene resources for breeding resistance varieties to Fusarium wilt of sea island cotton and its mechanism.[Method]Sea Island cotton resistance to Fusarium wilt disease 06-146,the susceptible materials of Xinhai 14 and the parents of the hybrid RIL high generation materials,10893 (super resistant),10895 (supper resistant),10879 (sensible)and 10796 (susceptible)were chosen as the experi-mental materials and at the same time,inoculation treatment was carried out.The seedlings of 0 h,4 h,10 h, 18 h,h,28,40 h,respectively,were taken to extract the mRNA and went through reverse transcription. Based on the known EST sequences of Fusarium wilt,resistance in upland cotton and the resistance related gene sequences screened from the Sea Island cotton disease resistance gene sequences,the real-time fluores-cence quantitative PCR reaction was carried out.According to the expression of different genes in different ma-terials,the relationship between different genes and the resistance to Fusarium wilt of cotton were analyzed.[Result]Four genes,CFW3,CFW10,comp62810,comp71372 in cotton may play a more important role in the process of fighting against Fusarium wilt of Sea Island cotton.In particular,the comp62810 and comp71372 gene,which were obtained from the transcriptional group of the disease resistant material and the susceptible material,were related to the disease resistance.[Conclusion]Through the analysis,it is conclu-ded that the sensitivity of different cotton varieties to the infection of the pathogen is different,which can be applied to determine the roles of the genes.关键词
海岛棉/枯萎病/抗性基因/实时荧光定量PCRKey words
cotton (Gossypium barbadense L.)/Fusarium wilt/real-time PCR分类
农业科技引用本文复制引用
杜荣光,姚正培,陈全家,张杰,杜丽丽,苏秀娟,曲延英..海岛棉抗枯萎病相关基因实时荧光定量PCR分析[J].新疆农业科学,2016,53(11):1980-1987,8.基金项目
国家自然科学基金项目（31260361）@@@@Supported by National Natural Science Foundation of China （）
