新疆农业科学2016，Vol.53Issue(11)：2069-2076,8.DOI:10.6048/j.issn.1001-4330.2016.11.014
基于RAPD标记的新疆荒漠稻区稻水象甲遗传多样性分析
Analysis on Genetic Diversity of the Rice Water Weevil Based on RAPD Markers in the Ecological Regions of Oasis in Xinjiang
摘要
Abstract
Objective]The objective of this experiment is to study the genetic structure and invasive route of L.oryzophilus in the hope of providing references that might be important for accurate monitoring and effective control of rice weevils.[Method]The L.oryzophilus were collected from 7 different geographic popu-lations of 105 individual from Xinjiang and north typical rice planting areas in China which was analyzed by u-sing the RAPD molecular markers in order to investigate the genetic diversity of the populations.[Result]7 good polymorphic RAPD primers that was finally-screened from 28 the random primers produced a total of 139 bands,of which 98 were polymorphic.The Polymorphism loci percentage(PPB)was 70.5%.The genet-ic diversity of the XJYNQ -apqal populations was the lowest (PPB:46.76%,H:0.210,6,I:0.323,9), while HLJAC was the highest (PPB:62.58 %,H:0.300,6,I:0.445,0)The coefficient of genetic differentia-tion among populations Gst was 0.199,5,including 19.95%genetic variation partitioned among populations, 80.05% within populations.It was showed higher levels of genetic differentiation between different geographic populations.7 geographical populations of UPGMA clustering results showed that:Tangshan City in Hebei Provice,Gongzhuling in Jilin Province,Hebei City,the geographical population is a priority,which indicated the close genetic relationship.Followed by are Acheng City in Heilongjiang Provice and Qapqal and Huocheng County in Yili area.These places completed the first cluster,while the other populations in Wujiaqu city and Midong district constituted the second cluster.[Conclusion]The richness of genetic diversity of the L.oryzo-philus different geographic populations is different and have genetic difference in China.The rice water weevil, L.oryzophilus Kuschel in the ecological regions of oasis in Xin-jiang were probably invaded from Northeast rice planting areas in China in the course of introduction,such as straws and traffic tools by the people.关键词
稻水象甲/RAPD/遗传分化Key words
Lissorhoptrus oryzophilus Kusche/RAPD/genetic differentiation分类
农业科技引用本文复制引用
王小武,付开,丁新华,吐尔逊·阿合买提,何江,郭文超..基于RAPD标记的新疆荒漠稻区稻水象甲遗传多样性分析[J].新疆农业科学,2016,53(11):2069-2076,8.基金项目
国家重点研发计划项目“主要入侵生物的动态分布与资源库建设”（2016YFC1202100）；自治区公益性科研院所基本科研业务费专项项目“稻水象甲监测及绿色防控技术研究”（KY2015061）；新疆农业科学院青年基金项目“稻水象甲在新疆的适生性分析及遗传多样性研究”（xjnkq－2014003）@@@@The national key research project ＂The dynamic distribution and resource database construction of the main invading organisms＂（2016YFC1202100）；the basic science and technology research support funds of non－profit research institutions of Xinjiang Uygur Autonomous Region ＂rice water weevil monitoring and control techniques of green control technology research＂（KY2015061），the Youth Funds of Xinjiang A-cademy of Agricultural Sciences ＂rice water weevil adaptability and genetic analysis in Xinjiang diversity＂ （）
