新疆农业科学2016，Vol.53Issue(11)：2077-2082,6.DOI:10.6048/j.issn.1001-4330.2016.11.015
应用实时荧光PCR检测甜菜霜霉病菌
Detection of Peronospora farinosa sp.betea Byford by Real-time PCR
摘要
Abstract
Objective]The objective of this study is to develop a SYBR GreenⅠreal-time PCR for the detection of Peronospora farinosa f.sp.betea Byford rapidly.[Method]The real-time PCR primers were de-signed based on the reported 28S rDNA gene sequences of P.farinosa sp.betea Byford and similar species. Totally 6 pathogens of beet and other 5 pathogens and 12 samples of beet seeds were screened to test the speci-ficity and sensitivity and applicability in this assay.[Result]The SYBR GreenⅠreal-time PCR assay could detect positive amplification from P.farinosa f.sp.betea Byford only,negative results from other pathogens and negative control,and more than 100 fg genomic DNA of P.farinosa sp.betea Byford could be detected. The 12 samples of beet seeds from different sources were detected by this assay,suggesting that the results were consistent with the field monitoring results.[Conclusion]This assay can detect P.farinosa sp.betea By-ford rapidly,and offer a technology to early diagnosis and control P.farinosa sp.betea Byford.关键词
甜菜霜霉病菌/实时荧光PCR/检测Key words
Peronospora farinosa sp.betea Byford/Real-Time PCR/detection分类
农业科技引用本文复制引用
张娜,乾义柯,魏霜,张维,焦子伟,张祥林..应用实时荧光PCR检测甜菜霜霉病菌[J].新疆农业科学,2016,53(11):2077-2082,6.基金项目
伊犁师范学院科研项目（2013YSYB13）；国家公益性行业（质检）科研项目（201310091）@@@@Supported by science and technology research projects of Yili Teachers＇University （2013YSYB13）and national public welfare industry （quality control）research project （）
