新疆农业科学2016，Vol.53Issue(11)：2149-2156,8.DOI:10.6048/j.issn.1001-4330.2016.011.025
北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析
PRRSV ORF7 Cloning and Genetic Evolution Analysis of Pigs in Northern Xinjiang
摘要
Abstract
Objective]To study porcine reproductive and respiratory syndrome virus (PRRSV)genetic variation to monitor the prevalence of PRRSV in Xinjiang and provide assistance to PRRSV serology.[Method]We used RT-PCR method for the separation of 18 PRRSV ORF7 gene which was amplified,cloned and se-quenced to analyze the ORF7 gene variation[Results]The 18 PRRSV ORF7 sequence mutation type belonged to North America type.They had different genetic strain relations with the international standards and national standards strains.XJzx 4 and XJzx 1 had a closer genetic relationship with the international standard strains 01NP1.2,PA8,VR-2332 and LMY.Except XJzx1,XJzx14,the rest strains had close relationships with the mainland strains BJsy06,GD,HB-1sh 2002,HuN,JXA1,LN,NX06 and SX2009,so it could be inferred that they evolved from the strains in mainland China.[Conclusion]It is hypothesized that local pop PRRSV strains are derived from different regions.We have not detected PRRSV ORF7 gene deletion mutation in Xin-jiang.关键词
猪繁殖与呼吸综合征病毒/RT-PCR/ORF7/序列分析Key words
PRRSV/RT-PCR/ORF7/sequence analysis分类
农业科技引用本文复制引用
吴鹏,张勋,梁田,陈新凯,肖媛媛,盛金良..北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析[J].新疆农业科学,2016,53(11):2149-2156,8.基金项目
国家自然科学基金项目“绵羊新抗微生物多肽基因的筛选、酵母表达及抗性分析”（30960276）；国家自然科学基金项目“新疆不同品种绵羊抗菌肽NK－Lysin多态性与抗肿瘤活性关系研究”（31360589）@@@@Supported by NSFC ＂Sheep new antimicrobial peptide gene screening，yeast expression and resistance analysis＂（30960276），NSFC ＂Relationship between NK－Lysin polymorphsim and antitumour activity in different breeds of sheep in Xinjiang＂ （）
