中风与神经疾病杂志2016，Vol.33Issue(11)：1017-1020,4.
骨髓间充质干细胞移植对大鼠脑缺血再灌注损伤的神经保护作用
Intravenous bone marrow-derived mesenchymal stem cells for the treatment of ischemia-reperfusion injury in rats
摘要
Abstract
Objective BMSCs were isolated in vitro and purified .Methods 10 mg/L BrdU was used for labeling before BMSCs transplantation .SD rats were used to establish the middle cerebral artery ischemia-reperfusion model with modified suture method .The models were randomly divided into transplantation group A、transplantation groupB and control groupC.7 days after injury,rats in group A were given 3 ×106 BMSCs,and in group B were given 1 ×106 BMSCs.Rats in the model group were left intact .Results Aafter transplantation of the BMSCs ,group A’ s recovery of the neurological def-icit is was more apparently than group C and group B (P<0.01).There was no significant difference between group B and group C (P>0.05).Double-staining cells of immunohistochemistry could be found at the center of damage and surround -ing of it.BrdU+cells and Immunohistochemical double staining positive cells were seen in group A and group B .BMSCs transplanted through vena caudalis can could survive and differentiate in the host brain .Conclusion BMSCs transplanted through vena caudalis can survive and differentiate in the host brain damage region .BMSCs transplantation is able to im-prove the neural function of cerebral ischemia injured rats. The efficacy is related to the number of BMSCs .关键词
骨髓间充质干细胞/大脑中动脉栓/尾静脉/大鼠/细胞移植Key words
Bone marrow-derived mesenchymal stem cells/MCAO/Vena caudalis transplantation/SD rats/Cell transplantation分类
临床医学引用本文复制引用
汪皖君,管雅琳,王雅静,于长申,刘首峰,吕凤琼,王世民,王新平..骨髓间充质干细胞移植对大鼠脑缺血再灌注损伤的神经保护作用[J].中风与神经疾病杂志,2016,33(11):1017-1020,4.基金项目
天津市科学技术委员会资助项目（No．11JCZDJC19400）；天津市卫生局立项课题 （）
