中国当代儿科杂志2016，Vol.18Issue(12)：1211-1216,6.DOI:10.7499/j.issn.1008-8830.2016.12.003
儿童急性髓细胞白血病WT1基因表达及其临床意义
Expression of WT1 gene in children with acute myeloid leukemia
摘要
Abstract
Objective To study WT1 gene expression in children with acute myeloid leukemia (AML) and its possible correlations to clinical outcomes. Methods Bone marrow samples were collected from 45 children with AML (excluding acute promyelocytic leukemia, AML-M3) at different time points of AML treatment and follow-up. WT1 gene expression levels in bone marrow mononuclear cells were assayed by real-time lfuorescence quantitative PCR. The correlation between WT1 expression and prognosis was retrospectively analyzed. Results The WT1 expression level in AML children with bone marrow blast cell percentage of >60% was significantly higher than in those with bone marrow blast cell percentage of≤60%(P<0.05). The lower WT1 expression level was documented in children with AML-M2 compared with in children with other non-M2 subtypes (P<0.05). WT1 expression level in patients in complete remission was signiifcantly lower than that in patients at diagnosis or relapse (P<0.01). The 2-year disease-free survival (DFS) in patients with higher WT1 expression was significantly lower than in those with lower WT1 expression at the end of induction chemotherapy (P<0.05). The 2-year overall survival (OS) and DFS in patients with≥1 log WT1 reduction range were signiifcantly higher than those with<1 log reduction of WT1 expression level at the end of induction chemotherapy (P<0.05). WT1 expression levels tended to rise 2-3 months prior to bone marrow relapse. Conclusions WT1 expression level is closely correlated prognosis in children with AML. Dynamic monitoring of WT1 expression level is of great clinical importance in terms of individualized management, prognosis evaluation and relapse prediction.关键词
急性髓细胞白血病/WT1基因/预后/实时荧光定量PCR/儿童Key words
Acute myeloid leukemia/WT1 gene/Prognosis/Real-time lfuorescence quantitative PCR/Child引用本文复制引用
唐雪,郭霞,杨雪,肖慧,孙静静,袁粒星,高举..儿童急性髓细胞白血病WT1基因表达及其临床意义[J].中国当代儿科杂志,2016,18(12):1211-1216,6.基金项目
四川省应用基础研究计划项目（2015JY0044）。 （）
