中国肺癌杂志2016，Vol.19Issue(12)：799-804,6.DOI:10.3779/j.issn.1009-3419.2016.12.01
自噬对人肺腺癌A549细胞放疗敏感性的影响
Role of Autophagy in the Radiosensitivity of Human Lung Adenocarcinoma A549 Cells
摘要
Abstract
Background and objective Radiotherapy is an important treatment for lung cancer. The poor prognosis of lung cancer is largely caused by the high recurrence rate and metastasis of the tumor. Autophagy, which can be induced by radiotherapy, might be associated with DNA repair. The aim of this study is to investigate whether activating autophagy us-ing rapamycin can enhance the radiosensitivity of lung cancer cells and clarify the association of autophagy with DNA repair. Methods The human adenocarcinoma A549 cell line was selected as the experimental subject. The specimens were divided into three groups: control (N), radiation (R), and Rapamycin and radiation (R+ARPA). hTe protein levels of γ-H2AX, Rad51, Ku70/Ku80, p62, and LC3 were determined by Western blot. Autophagosome was observed under a transmission electron microscope, and SF was determined by colony formation assay.Results Compared with group R, the activity of autophagy and the protein expression levels of Rad51 and Ku70/80 were remarkably increased in group R+ARPA.ConclusionhTe radiosensitivity of lung cancer can be promoted by activating autophagy via treatment with Rapamycin, and the process may be associated with DNA repair.关键词
自噬/肺肿瘤/DNA损伤修复/放疗敏感性Key words
Autophagy/Lung neoplasms/DNA repair/Radiosensitivity引用本文复制引用
徐丽瑶,王勇,刘琴,罗辉,钟小军,李勇..自噬对人肺腺癌A549细胞放疗敏感性的影响[J].中国肺癌杂志,2016,19(12):799-804,6.基金项目
本研究受国家自然科学基金（No.81560379）、江西省科技支撑计划项目（No.2015BBG70236）及江西省教育厅科学技术研究项目（No.14025）资助@@@@ This study was supported by the grants from National Natural Science Foundation of China (No.81560379), Science and Technology Supporting Program of Jiangxi Province (No.2015BBG70236) and Science and Technology Research Project of Education Department of Jiangxi Province (No.14025)(All to Yong LI) （No.81560379）
