中国肺癌杂志2016，Vol.19Issue(12)：805-812,8.DOI:10.3779/j.issn.1009-3419.2016.12.02
Amorphigenin联合顺铂对人肺腺癌A549/DDP细胞的协同抗肿瘤作用
Synergistic Antitumor Effect of Amorphigenin Combined with Cisplatin in Human Lung Adenocarcinoma A549/DDP Cells
摘要
Abstract
Background and objective Amorphigenin, a rotenoid compouns, from seeds of Amorpha fruticosa, has been shown to possess anti-proliferation activities in several cancer cells. To explore the antitumor effects of amorphigenin on cisplatin-resistant human lung adenocarcinoma A549/DDP cells and explore the underlying mechanisms.Methods CCK-8 assay was used to measure the proliferation of A549/DDP cells; Colony formation assay was used to measure the colony formation of A549/DDP cells; Flow cytometry assay was used to detect the apoptosis rates; Western blot analysis was used to explore the expression of apoptosis-related proteins (caspase-3 protein, PARP protein) and lung resistance protein (LRP). Results Our results demonstrated that amorphigenin could inhibit the proliferation of A549/DDP cells with a inhibition con-centration of 50% cell growth (IC50) at 48 h of (2.19±0.92) μmol/L. Amorphigenin could inhibit the colony formation ability and induce apoptosis of A549/DDP cells; Furthermore, amorphigenin combined with cisplatin showed synergistic prolifera-tion-inhibitory effect and apoptosis-promoting effect in A549/DDP cells; reduced the expression of LRP of A549/DDP cells. Conclusion Amorphigenin remarkably inhibits the proliferation and induces apoptosis in A549/DDP cells. Combination of amorphigenin with cisplatin had the synergistic inhibitory effect on A549/DDP cells by downregulating the expression of LRP.关键词
Amorphigenin/顺铂/肺腺癌/凋亡/抗肿瘤作用Key words
Amorphigenin/Cisplatin/Lung adenocarcinoma/Apoptosis/Antitumor effect引用本文复制引用
钟红珍,左瑜芳,巫鑫,彭艳,何会萍,杨俊,官成浓,徐祖敏..Amorphigenin联合顺铂对人肺腺癌A549/DDP细胞的协同抗肿瘤作用[J].中国肺癌杂志,2016,19(12):805-812,8.基金项目
本研究受国家自然科学基金（No.81201736）和广东省科技计划项目（No.2010B031600289）资助@@@@ This study was supported by the grants from the National Nature Science Foundation of China (to Zumin XU)(No.81201736) and the Science and Technology Planning Project of Guangdong Province (to Chengnong GUAN)( No.2010B031600289) （to Zumin XU）
