中国免疫学杂志2016，Vol.32Issue(12)：1729-1733,5.DOI:10.3969/j.issn.1000-484X.2016.12.001
RAC1基因多态性对Rac1-GTP蛋白表达水平的影响
Influence of RAC1 gene polymorphisms on Rac1-GTP expression levels
摘要
Abstract
Objective:To study the association of RAC1 gene polymorphisms with protein expression levels of Rac1-GTP. Methods:A total of 182 healthy Hans population in Hubei were recruited. The 4 tag-SNPs in RAC1 gene were genotyped by Real time TaqMan-MGB genotyping assay. And the Rac1-GTP protein levels in plasma samples from all participants were determined by enzyme linked immunosorbent assays ( ELISA ) . The distribution characteristics of Rac1-GTP expression levels were also analyzed. Furthermore,the expression levels of Rac1-GTP were compared among different genotypes of the 4 tag-SNPs in RAC1 gene. Results:The distribution of Rac1-GTP expression levels was positive skewed in healthy Chinese Hans population. The expression levels were significantly higher in females than in males (P<0. 05),and appeared in decreased trend with age,but without significant differences (P>0. 05). Different expression levels of Rac1-GTP were observed in different genotypes for rs702482 and rs10951982 (P<0. 05). However,no significant difference was found for rs702483 and rs6954996 (P>0. 05). Conclusion:RAC1 genetic polymorphisms can potentially affect the expression levels of Rac1-GTP protein in healthy Chinese Hans population.关键词
RAC1/基因多态性/Rac1-GTP/蛋白表达水平/汉族健康人群Key words
RAC1/Gene polymorphisms/Rac1-GTP/Protein expression levels/Healthy Chinese Hans population分类
医药卫生引用本文复制引用
刘亚妮,周嘉黎,杨春晓,罗小梅,杨婷玉,张玉,师少军,黄怡菲..RAC1基因多态性对Rac1-GTP蛋白表达水平的影响[J].中国免疫学杂志,2016,32(12):1729-1733,5.基金项目
本文为国家自然科学基金( No.81273591、No.81503161)、湖北省自然科学基金(No.2009CD-B380)和中央高校基本科研业务费专项资金(No.2014-YGYL003)资助项目。 （ No.81273591、No.81503161）
