中国药理学与毒理学杂志2016，Vol.30Issue(11)：1172-1175,4.DOI:10.3867/j.issn.1000-3002.2016.11.007
达肝素钠和肝素钠对小鼠毒性的比较
Comparison of toxicity of dalteparin sodium and heparin sodium of mice in vivo and in vitro
张媛 1纳涛 2吴彦霖 1游赣花 3刘倩 1贺庆 1高华1
作者信息
- 1. 中国食品药品检定研究院药理室，北京 100050
- 2. 中国食品药品检定研究院细胞资源保藏研究中心，北京 100050
- 3. 贵州食品药品检验所药理室，贵州贵阳 550004
- 折叠
摘要
Abstract
OBJECTIVE To investigate the toxicity of dalteparin sodium and heparin sodium in vitro and in vivo. METHODS The median lethal dose(LD50)of these two drugs in mice was determined. The proliferation activity of L-929 cells treated with dalteparin sodium(0.78×105-5.00×106 U·L-1)or heparin sodium(0.39×105-1.25×106 U·L-1)for 48 h was detected by CCK8 kit. The half maximal inhibitory concen⁃tration(IC50)of L-929 cells was calculated. The cell cycle was analyzed by flow cytometry. RESULTS LD50 of mice with acute toxicity induced by dalteparin sodium or heparin sodium was 4.44×105 and 3.68× 105 U·kg-1,respectively. IC50 of L-929 cells treated with them was 1.42×106 and 3.54×105 U·L-1,respec⁃tively. The percentage of G2/M-phase (137.6 %,P<0.01) and G0/G1-phase (33.8%,P<0.01) cells was significantly increased after the treatment with dalteparin sodium or heparin sodium. CONCLUSION The toxicity of dalteparin sodium in vivo and in vitro is not so strong as that of heparin sodium. Dalteparin sodium and heparin sodium arrest L-929 cells at G2/M-phase and G0/G1-phase,respectively.关键词
达肝素钠/肝素钠/急性毒性/细胞毒性/细胞周期Key words
dalteparin sodium/heparin sodium/acute toxicity/cytotoxicity/cell cycle分类
医药卫生引用本文复制引用
张媛,纳涛,吴彦霖,游赣花,刘倩,贺庆,高华..达肝素钠和肝素钠对小鼠毒性的比较[J].中国药理学与毒理学杂志,2016,30(11):1172-1175,4.
