中国医学创新2016，Vol.13Issue(32)：18-21,4.DOI:10.3969/j.issn.1674-4985.2016.32.005
乌司他丁对机械通气相关性肺损伤模型兔肺组织中TNF-α的影响
Effect of Ulinastatin on TNF-α Mechanical Ventilation Induced Lung Injury in Rabbit Model of Lung Tissue
摘要
Abstract
Objective:The establishment of ventilator induced lung injury in a rabbit model of lung tissue, to observe the changes of TNF-α in mechanical ventilation induced lung injury model of rabbit lung tissue alpha, to explore the therapeutic mechanism of Ulinastatin on VILI and TNF-α.Method:The injury rabbit model of high tidal volume ventilation was established,after 30,60,120,180 and 240 min,arterial blood for blood gas analysis.TNF-α in serumwere measured by ELISA.The pathological changes of lung tissue were observed by HE staining.The expression of TNF-αRNA and protein in lung tissue was detected by PCR Real-time and Blot Western.Result:After mechanical ventilation,the expression of TNF-α in serum and lung tissue increased significantly.Ulinastatin interved ventilator induced lung injury model in rabbits and rabbit arterial blood PaO2 increased,in serum and lung tissue TNF-α expression were compared with those of the earlier high tidal volume group improved significantly(P<0.05).Conclusion:Ulinastatin on ventilator induced lung injury has a protective effect, he mechanism may be by down regulating the expression of TNF-α,inhibit inflammatory reaction to achieve.关键词
乌司他丁/呼吸机相关性肺损伤/大潮气量/TNF-αKey words
Ulinastatin/Ventilator associated lung injury/Tidal volume/TNF-α引用本文复制引用
闵军,潘定斌,雷霆,林萍清,佘晖,董芳..乌司他丁对机械通气相关性肺损伤模型兔肺组织中TNF-α的影响[J].中国医学创新,2016,13(32):18-21,4.基金项目
福州市科技计划项目 （）
