中国药业2016，Vol.25Issue(23)：68-70,3.
高效液相色谱－电化学检测器测定富硒酵母中硒代蛋氨酸含量
Determination of Selenomethionine in Selenium-Enriched Yeast by HPLC-Electrochemical Detector
摘要
Abstract
Objective To establish an HPLC-electrochemical detection method for the determination of selenomethionine in selenium-enriched teast. Methods The column was an Agilent Eclipse plus C18 column ( 150 mm × 3. 0 mm, 3. 5 μm ) . 100 mmol/L citrate buffer ( pH=2. 75, containing 300 mg/L1-Octanesulfonic acid sodium salt ) -methanol ( 85 : 15 ) was used as the mobile phase and the detection voltage was set at 500 mV ( ECD1 ) and 700 mV ( ECD2 ) . Results Selenomethionine showed good linearitt in the range of 1-300 μmol/L ( r2=0. 999 6 ) , the detection limit was 0. 30 μmol/L, and the recovert rate was 101. 30%, RsD was 2. 30% ( n=6 ) . Conclusion The method is simple, sensitive, reproducible, which can be used for the determination of selenomethionine in selenium-enriched teast.关键词
高效液相色谱-电化学检测器/富硒酵母/硒代蛋氨酸Key words
HPLC-electrochemical detector/selenium-enriched teas/selenomethionine分类
医药卫生引用本文复制引用
陈荣祥,保玉心..高效液相色谱－电化学检测器测定富硒酵母中硒代蛋氨酸含量[J].中国药业,2016,25(23):68-70,3.基金项目
遵义医学院博士启动基金，项目编号F－798；贵州省科技厅基础研究项目，项目编号黔科合基础〔2016〕1172。 （）
