摘要
Abstract
Objective To explore the effect of arun doin on cell apoptosis in human prostate cancer LNCap cells and the mechanism. Methods LNCap cells were treated with arundoin in different concentration. The relative cell viabilities were determined by the MTT method, the morphological changes of cells were observed using Hoechst 33258 staining, and the apoptosis and cell cycle were analyzed by flow cytometry. At the same time, the protein expressions of Bcl-2, Bax, caspase-3 and caspase-9 were detected by Western blot. Re⁃sults Arundoin (20-120 μg/mL) significantly inhibited the viabilities of LNCap cells in a dose-dependent man⁃ner. Hoechst 33258 staining showed that arundoin increased the membrane permeability of LNCap cells. Flow cy⁃tometry results showed that arundoin could induce apoptosis in LNCap cells, the apoptotic ratios were 11.3% (20μg/mL), 25.2% (40 μg/mL), 57.1% (80 μg/mL), significantly higher than that of control group 0.5% (0 μg/mL). After different concentrations of arundoin treatment for 48 h, the proportion of LNCap cells in G0/G1 phase was increased, while the proportion of the G2/M phase and S phase were reduced. In 80 μg/mL arundoin treatment group, the proportion of cells in G0/G1 phase was increased significantly. The data of Western blot showed that arundoin (20, 40, 80 μg/mL) up-regulated the expression levels of cleaved caspase-3, cleaved caspase-9, and Bax, but down-regulated Bcl-2, in a dose-dependent manner. Conclusion Arundoin could inhibit the prolif⁃eration of LNCap cells and promote apoptosis, which may be associated with the down regulation of Bcl-2 ex⁃pression and up regulation of Bax expression, as well as the increase of relative activity of caspases.关键词
前列腺癌/芦竹素/凋亡/Bcl-2家族蛋白Key words
Prostate cancer/arun doin/apoptosis/Bcl-2 family protein分类
医药卫生
