中国中医药信息杂志2017，Vol.24Issue(1)：23-27,5.DOI:10.3969/j.issn.1005-5304.2017.01.007
痰湿质酒精性股骨头坏死CYP2C8基因 rs17110453位点多态性研究
Study on Alcohol-induced Osteonecrosis of Femoral Head of Phlegm-dampness Constitution with CYP2C8 Gene Polymorphism
摘要
Abstract
Objective To study the relation between alcohol-induced osteonecrosis of femoral head (AIONFH) related with high morbidity TCM constitution type with CYP2C8 gene polymorphisms.Methods Totally 152 Han nationality NONFH cases from Feburary 2014 to September 2015 from outpatient and the inpatient departments in Gansu Province Hospital of TCM were collected. 50 AIONFH cases were set as medical case group; meanwhile, 45 healthy volunteers were enrolled as control group. Database for medical materials of all patients and volunteers was established. TCM distribution for AIONFH patients was determined. Solution DNA extraction kit was used to extract DNA, and detect the concentration and purity of DNA. The target gene was amplified by PCR and the target gene was amplified by gel electrophoresis. The length of the fragment was confirmed to conduct target gene sequencing. With the results of sequencing and gel electrophoresis, the relation of AIONFH with CYP2C8 gene polymorphism in AIONFH patients with phlegm-dampness syndrome and the control group.ResultsThe CYP2C8 gene loci rs17110453 gene polymorphism was not statistically significant between the two groups (χ2=0.253,P>0.05). There was no significant difference in allele between the two groups (χ2=0.077,P>0.05). The risk of disease in CC genotype was 1.37 times higher than the AA genotype (95%CI: 0.339-5.540), without statistical significance (P>0.05). There was no significant difference in genotype and allele distribution between AIONFH patients with phlegm-dampness and non-phlegm-dampness and the control group (P>0.05).Conclusion CYP2C8 gene loci rs17110453 gene polymorphism A/C mutation has no obvious relation with AIONFH risk. There is no clear relationship between CYP2C8 gene loci rs17110453 gene polymorphism with AIONFH.关键词
酒精性股骨头坏死/中医体质/痰湿质/基因多态性Key words
alcohol-induced osteonecrosis of femoral head/TCM constitution/phlegm-dampness constitution/gene polymorphism分类
医药卫生引用本文复制引用
柳海平,周明旺,李盛华,陈威,叶丙霖,郭铁峰,陈娴..痰湿质酒精性股骨头坏死CYP2C8基因 rs17110453位点多态性研究[J].中国中医药信息杂志,2017,24(1):23-27,5.基金项目
国家自然科学基金（81473712）；甘肃省自然科学基金 （）
