中国中医药信息杂志2017，Vol.24Issue(1)：49-54,6.DOI:10.3969/j.issn.1005-5304.2017.01.013
复方龙芩草微乳提取液有效成分体外抗H1N1流感病毒的药效研究
Study on Anti-influenza Virus H1N1 Activity of Main Ingredients from Microemulsion Extract of Compound Longqincaoin Vitro
摘要
Abstract
Objective To study the effects of main ingredients from microemulsion extract of Compound Longqincao in vitro on anti-influenza virus H1N1 activity; To analyze effects of main ingredients from microemulsion extract on influenza virus inhibition rate.Methods Uniform design was used to conduct the experiment. MTT method was used to detect the effect rate (ER) of anti-influenza virus H1N1 on A549 cells. Setting ER as the index, Minitab17 software was used to establish mathematical model to come up with regression equations of all factors. The effects of ingredients on ER were analyzed and the efficient composition ratio of the optimum anti-influenza virus H1N1 was chosen.ResultsIn the compound compatibility, baicalin showed the most obvious antivirus activity, and licorice glycosides had certain inhibition effects on pathological changes of cells. Five ingredients had coordinative or controlled relation with ER. When per milliliter liquids containing licorice glycosides, baicalin, leucine, aspartic acid, glutamic acid was 13.94μg, 49.44μg, 0.23 mg, 1.25 mg, and 2.50 mg, ER was the best. ER was 85.34%±4.72% after verification.ConclusionThe optimized combination of main ingredients from microemulsion extract of Compound Longqincaocan better play a role in anti-influenza virus H1N1.关键词
复方龙芩草/微乳提取液/均匀设计/H1N1流感病毒/A549细胞Key words
CompoundLongqincao/microemulsion extract/uniform design/H1N1 influenza virus/A549 cells分类
医药卫生引用本文复制引用
刘晓艳,李妍,刘帅,李卫飞,顾立刚,刘晓婷,杜红..复方龙芩草微乳提取液有效成分体外抗H1N1流感病毒的药效研究[J].中国中医药信息杂志,2017,24(1):49-54,6.基金项目
国家自然科学基金(81102815)；教育部博士点新教师基金(20110013120017)；北京中医药大学研究生自主课题(2016-JYB-XS069) （81102815）
