中国中医药信息杂志2017，Vol.24Issue(1)：87-90,4.DOI:10.3969/j.issn.1005-5304.2017.01.021
非生物诱导子茉莉酸甲酯和水杨酸对半夏悬浮细胞中生物碱代谢的影响
Effects of Abiotic Elicitors MeJA and SA on Alkaloids Accumulation and Related Enzymes Metabolism inPinellia ternata Suspension Cell Cultures
摘要
Abstract
Objective To study the effects of abiotic elicitors methyl jasmonate (MeJA) and salicylic acid (SA) on the alkaloids accumulation and related enzymes metabolism inPinellia ternata suspension cell cultures. Methods Using the leaf petioles-derived suspension cell cultures as the study object, the culture duration, concentrations of MeJA and SA were determined to get the optimal alkaloids accumulation, and the activities of metabolic enzymes IMP dehydrogenase and sAMP synthase were also measured.Results A 9-fold of dried biomass and a 3-fold of alkaloids accumulation were observed inP. ternata suspension cell cultures after culture for 21 d. Both MeJA and SA could significantly promote the accumulation of alkaloids inP. ternata suspension cells. 150 µmol/L MeJA enhanced alkaloids content (4.7 mg/gDW) by 3.6 folds in comparison with control group, whereas 50 µmol/L SA showed a 2.5-fold increase. Meanwhile, 100 µmol/L MeJA and 50 µmol/L SA promoted the increase in IMP dehydrogenase activity by 3.0 and 3.7 fold respectively, and 150 µmol/L MeJA and 100 µmol/L SA showed the increase by 2.6 and 4.4 fold respectively.Conclusion Proper adding exogenous MeJA and SA can promote the accumulation of alkaloids inPinellia ternata suspension cell cultures.关键词
半夏/悬浮细胞/生物碱/茉莉酸甲酯/水杨酸Key words
Pinellia ternata/suspension cell cultures/alkaloids/methyl jasmonate/salicylic acid分类
医药卫生引用本文复制引用
段永波,鲁放,崔婷婷,赵丰兰,滕井通,盛玮,张爱民,薛建平..非生物诱导子茉莉酸甲酯和水杨酸对半夏悬浮细胞中生物碱代谢的影响[J].中国中医药信息杂志,2017,24(1):87-90,4.基金项目
国家自然科学基金（31501368、81573518）；安徽省自然科学基金（1408085MC58、1608085MC52）；安徽高校省级自然科学研究项目 （）
