神经药理学报2016，Vol.6Issue(1)：18-24,7.DOI:10.3969/j.issn.2095-1396.2016.01.003
2’-溴-2-羟基-4，6，4’，5’-四甲氧基查尔酮抑制SK-N-SH细胞增殖的作用研究
Antiproliferation Effects of2’-bro-2-hydroxy-4,6,4’,5’-tetrametho-xychalcone on Human Neuroblastoma SK-N-SH Cells
摘要
Abstract
[ABSTRACT]Objective:We studied the anti-proliferation effects of 2’-bro-2-hydroxy-4, 6,4’,5’-tetrametho-xychalcone(C30) on human neuroblastoma and its mechanism to provide preliminary experimental evidence for its potential application to the prevention and treatment of neuroblastoma.Methods:The antiproliferation effects of the compounds on tumor cells were detected by sulforhodamine B(SRB) assay. Cell cycle distributions were measured by flow cytometry. Cellular apoptosis was detected by Hoechst 33258 staining assay and flow cytometry. Results:C30 restrained the proliferative effect of SK-N-SH cells in a dose- and time- dependent manner. After treated with C30,the cell cycle of SK-N-SH was arrested in G1 phase. Hoechst 33258 staining and flow cytometry analysis revealed that petasin could induce apoptosis in SK-N-SH cells.Conclusion:C30can inhibit the proliferation of SK-N-SH,and the mechanism may be related to changing cell cycle distribution and inducing apoptosis.关键词
人神经母细胞瘤/查尔酮/细胞增殖/细胞周期/凋亡Key words
neuroblastoma/chalcone/cell proliferation/cell cycle/apoptosis分类
医药卫生引用本文复制引用
刘军举,付冬君,牛进波,张旗,张雁冰..2’-溴-2-羟基-4，6，4’，5’-四甲氧基查尔酮抑制SK-N-SH细胞增殖的作用研究[J].神经药理学报,2016,6(1):18-24,7.基金项目
国家自然科学基金资助项目 （）
