渔业研究2016，Vol.38Issue(6)：431-436,6.DOI:10.14012/j.cnki.fjsc.2016.06.001
网箱养殖沉积环境中硫氧化菌的分离鉴定及生长特性
Isolation, identification and characteristics of a sulfide-oxidizing bacterium in sediment of marine cage culture area
摘要
Abstract
Screening sulfide-oxidizing bacteria from sediments of marine cage culture area, then exploring the growth characterization of the bacteria. The medium with sodium thiosulfate as the sole energy substrate was used to isolate a sulfide-oxidizing bacterium. The species of the strain were identified by a variety of methods of Gram staining, plate colony morphologic observation, morphological characteristics research, 16S rRNA gene sequence analysis and the molecular phylogenetic tree construction. An efficient thiosulphate-oxidizing bacterium was isolated from Bachimen of Fujian, named B1 -1. The strain was Gram-negative bacteria, short rod and identified as Halothiobacillus hydrothermalis ( GenBank accession no. KU362926 ) . Under the culture conditions of 28°C, pH 8 and 150 r/min, the phases of logarithmic of strain B1 -1 had come in 12 h, then the thiosulfate was oxidated in 36 h, the medium pH was down to 4. 8 and the concentra-tion of S2 O2-3 was reduced from initial 2. 28 g/L to 0. 36 g/L in 60 h. The strain of sodium thiosulfate oxida-tion was up to 84. 2%, which showed great application prospect.关键词
网箱养殖/盐硫杆菌属/分离鉴定/硫代硫酸盐/生长特性Key words
cage culture/Halothiobacillus sp./isolation and identification/thiosulfate/growth character-istics分类
生物科学引用本文复制引用
陈小红,陈宇锋,郑惠东,陈月忠,许贻斌,陈财珍,罗冬莲..网箱养殖沉积环境中硫氧化菌的分离鉴定及生长特性[J].渔业研究,2016,38(6):431-436,6.基金项目
福建省公益类科研专项(2014R1003-12,2014R1003-3) （2014R1003-12,2014R1003-3）
国家海洋局海洋公益性行业科研专项(201305043-4) （201305043-4）
闽台重要海洋生物资源高值化开发技术公共服务平台(2014FJPT01) （2014FJPT01）
福建重要海洋经济生物种质库与资源高效开发技术公共服务平台(14PZY017NF17) （14PZY017NF17）
